人脐静脉内皮细胞HUVEC

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3D细胞大规模培养》

人脐静脉内皮细胞

英文名：Human Umbilical Vein Endothelial Cells
货号：8000
价格：￥5148.00

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人脐静脉内皮细胞说明：

血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究，人脐静脉和动脉内皮细胞是这类研究中常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”，VIII因子阳性着色，能摄取乙酰基化低密度脂蛋白，人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。

人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

配套培养基：内皮细胞培养基(Cat.No.1001)

产品使用说明：仅供科研研究使用

货号	8000
产地	美国
缩写	HUVEC
规格	5 x 10^5 cells/vial
用途	科研
运输	干冰
保存	液氮

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Question:
Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?

Answers:
Posted by Manoj Sharma on Thursday, August 20, 2015
Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.
Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research laboratories that use human tissues.” J Tissue Culture Methods. 11: 191-9
Please reply here if you have further questions
Best,
ScienCell Team

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