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原代细胞永生化

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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

指动物、人的原代细胞受到病毒感染、外源基因插入、辐射或药物作用后,

细胞分裂能力从有限的倍增次数转变为可以无限体外扩增的能力。该项技术的出

现极大的丰富了人们对于珍贵的、不能体外大量扩增细胞的来源。


三个方案可供选择:
















部分产品列表如下:


1 Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells
2 Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells
3 Conditionally Immortalized Human Adipose Tissue-Derived Mesenchymal Stem Cells
4 Conditionally Immortalized Human Adipose Tissue-Derived Mesenchymal Stem Cells
5 Conditionally Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells
6 Conditionally Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells
7 Conditionally Immortalized Human Retinal Pigment Epithelial Cells
8 Conditionally Immortalized Human Retinal Pigment Epithelial Cells
9 Conditionally Immortalized Human Neuron Cells
10 Conditionally Immortalized Human Neuron Cells
11 Conditionally Immortalized Human Liver Stellate Cells
12 Conditionally Immortalized Human Liver Stellate Cells
13 Conditionally Immortalized Rat Cardiac Microvascular Endothelial Cells
14 Conditionally Immortalized Rat Cardiac Microvascular Endothelial Cells




有关参考文献:

1. Jat PS, Sharp PA.Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts.J Virol. 1986 Sep;59(3):746-50.

 

2. Velcich A, Ziff E.Adenovirus E1a proteins repress transcription from the SV40 early promoter. Cell. 1985 Mar;40(3):705-16.

 

3. Strauss M, Griffin BE.Cellular immortalization--an essential step or merely a risk factor in DNA virus-induced transformation? Cancer Cells. 1990 Nov;2(11):360-5.

 

4. Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature. 1998 Nov 5;396(6706):84-8.

 

5. Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp PM, Sedivy JM, Weinberg RA. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14723-8.

 

6. Hahn WC, Counter CM, Lundberg AS, Beijersbergen RL, Brooks MW, Weinberg RA. Creation of human tumour cells with defined genetic elements.Nature. 1999 Jul 29;400(6743):464-8.

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