产品名称 |
NCI-H838人肺癌细胞 |
货号 |
ZQ0010 |
产品介绍 |
NCI-H838 [H838]是从患有3B腺癌阶段的59岁男性的肺中分离的呈现上皮形态学的细胞系。这株细胞建立于1984年10月,病人是一个吸烟者,每年吸烟80包。细胞表现出典型的上皮样形态,并在体外贴壁生长。NCI-H838细胞系在癌症研究中非常重要,因为它们保留了与原始肺癌组织相似的遗传基因组和生物学特性。 |
种属 |
人 |
性别/年龄 |
男/59岁 |
组织 |
肺;来源于转移部位:淋巴结 |
疾病 |
3B,腺癌;非小细胞肺癌 |
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X
CSF1PO: 10
|
细胞类型 |
肿瘤细胞 |
形态学 |
上皮样 |
生长方式 |
贴壁 |
倍增时间 |
大约26~55小时 |
培养基和添加剂 |
|
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC |
供应限制 |
仅供科研使用 |
货号 |
ZQ0010 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
1、PubMed=25714623; DOI=10.1371/journal.pone.0116218
Liang-Chu M.M.Y., Yu M.-M., Haverty P.M., Koeman J., Ziegle J., Lee M., Bourgon R., Neve R.M.
Human biosample authentication using the high-throughput, cost-effective SNPtrace(TM) system.
PLoS ONE 10:E0116218-E0116218(2015)
2、PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)
3、PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)
4、PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)
5、PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
6、PubMed=29444439; DOI=10.1016/j.celrep.2018.01.051
Yuan T.L., Amzallag A., Bagni R., Yi M., Afghani S., Burgan W., Fer N., Strathern L.A., Powell K., Smith B., Waters A.M., Drubin D.A., Thomson T., Liao R., Greninger P., Stein G.T., Murchie E., Cortez E., Egan R.K., Procter L., Bess M., Cheng K.T., Lee C.-S., Lee L.C., Fellmann C., Stephens R., Luo J., Lowe S.W., Benes C.H., McCormick F.
Differential effector engagement by oncogenic KRAS.
Cell Rep. 22:1889-1902(2018)
7、PubMed=29681454; DOI=10.1016/j.cell.2018.03.028
McMillan E.A., Ryu M.-J., Diep C.H., Mendiratta S., Clemenceau J.R., Vaden R.M., Kim J.-H., Motoyaji T., Covington K.R., Peyton M., Huffman K., Wu X.-F., Girard L., Sung Y., Chen P.-H., Mallipeddi P.L., Lee J.Y., Hanson J., Voruganti S., Yu Y., Park S., Sudderth J., DeSevo C., Muzny D.M., Doddapaneni H., Gazdar A.F., Gibbs R.A., Hwang T.H., Heymach J.V., Wistuba I.I., Coombes K.R., Williams N.S., Wheeler D.A., MacMillan J.B., DeBerardinis R.J., Roth M.G., Posner B.A., Minna J.D., Kim H.S., White M.A.
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)
8、PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
9、PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
10、PubMed=31803961; DOI=10.1002/jcb.29564
Mulshine J.L., Ujhazy P., Antman M., Burgess C.M., Kuzmin I.A., Bunn P.A. Jr., Johnson B.E., Roth J.A., Pass H.I., Ross S.M., Aldige C.R., Wistuba I.I., Minna J.D.
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)
11、PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)
12、PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0002 | 95-D [PLA-801D]人肺癌细胞(高转)[套餐促销] | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0015 | NCI-H1703人肺鳞癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0016 | NCI-H226人肺鳞癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0111 | NCI-H460人肺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0388 | NCI-H292人肺癌细胞(淋巴结转移) (STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0394 | Calu-1人肺癌细胞 (STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
3 | 细菌基因敲除 | ¥询价 | 放入购物车 》 | |
7 | 细胞基因敲除 | ¥询价 | 放入购物车 》 | |
ZQ-200 | RPMI-1640基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-201 | RPMI-1640完全培养基 | 500ml | ¥350.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |