产品名称 |
SK-N-AS人神经母细胞瘤细胞 |
货号 |
ZQ0051 |
产品介绍 |
SK-N-AS人神经母细胞瘤细胞是一种源自人类神经母细胞瘤的细胞系,最初从一名6岁患有低分化胚胎性神经母细胞瘤的女性患儿的骨髓转移病灶中分离得到。这些细胞具有上皮细胞样的形态,是贴壁生长的细胞类型,并且能够在体外培养条件下增殖。SK-N-AS人神经母细胞瘤细胞系在神经科学研究中具有多种应用,特别是在研究神经母细胞瘤的发病机制、药物反应和肿瘤转移方面。例如:研究神经母细胞瘤的骨转移、药物反应研究、表观遗传学研究、神经母细胞瘤的增殖和分化研究、神经母细胞瘤的病理学研究。这些研究不仅有助于理解神经母细胞瘤的复杂生物学特性,而且对于开发新的治疗方法和药物也具有重要意义。 |
种属 |
人 |
性别/年龄 |
女/6岁 |
组织 |
大脑;来源于转移部位:骨髓 |
疾病 |
神经母细胞瘤 |
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X
CSF1PO: 10,12
|
细胞类型 |
肿瘤细胞 |
形态学 |
上皮细胞 |
生长方式 |
贴壁 |
换液频率 |
2~3次/周 |
培养基和添加剂 |
DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%胎牛血清 (中乔新舟 货号:ZQ500-A)+1%P/S(货号:CSP006)+0.1 mM NEAA(中乔新舟 货号:CSP008) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2137 ECACC; 94092302 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0051 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
DOI=10.1159/000407025
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1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.
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NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
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PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
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Integrated genomic analyses identify ARID1A and ARID1B alterations in the childhood cancer neuroblastoma.
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PubMed=28350380; DOI=10.1038/sdata.2017.33; PMCID=PMC5369315
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)
PubMed=30459281; DOI=10.1126/scisignal.aar5680
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Phosphoproteome and gene expression profiling of ALK inhibition in neuroblastoma cell lines reveals conserved oncogenic pathways.
Sci. Signal. 11:eaar5680.1-eaar5680.16(2018)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)
PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)
PubMed=33921066; DOI=10.3390/cancers13081909; PMCID=PMC8071449
Siaw J.T., Gabre J.L., Uckun E., Vigny M., Zhang W.-C., Van den Eynden J., Hallberg B., Palmer R.H., Guan J.-K.
Loss of RET promotes mesenchymal identity in neuroblastoma cells.
Cancers (Basel) 13:1909.1-1909.25(2021)
PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0050 | SH-SY5Y人神经母细胞瘤细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0398 | IMR-32人神经母细胞瘤细胞 (STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0412 | SK-N-BE(2)人神经母细胞瘤细胞 (STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0413 | SK-N-SH人神经母细胞瘤细胞 | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
2 | 基因敲除小鼠 | ¥询价 | 放入购物车 》 | |
5 | 荧光素酶标记 | ¥询价 | 放入购物车 》 | |
6 | 绿/红色荧光蛋白标记 | ¥询价 | 放入购物车 》 | |
7 | 细胞基因敲除 | ¥询价 | 放入购物车 》 | |
ZQ-100 | DMEM(高糖)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-110 | DMEM(高糖)完全培养基 | 500ml | ¥500.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |