名称 |
C2C12小鼠胚胎肌母细胞 |
货号 |
ZQ0092 |
产品介绍 |
该细胞株是YaffeD,SaxelO建立的小鼠成肌细胞系的亚株。该细胞分化较快,可形成能收缩的微管,产生特异的肌肉蛋白。在骨形态形成蛋白(BMP-2)的作用下,该细胞可由成肌细胞分化为成骨细胞。检测发现该细胞鼠痘病毒阴性。 注意事项:C2C12细胞生长较快,密度达到80%即可传代。培养时细胞密度不能过高(细胞密度控制在 5000 – 15000 活细胞/平方厘米), 并应及时更换培养液,否则容易分化。 收到邮寄活细胞的,收集上清离心后收集脱落的悬浮细胞和贴壁细胞一起建议T25培养瓶1:2传代培养,参考传代比例根据细胞数量决定。传代周期2-3天,隔天换液。 |
种属 |
小鼠 |
组织来源 |
骨骼肌组织 |
形态学 |
成肌细胞样 |
细胞类型 |
自然永生细胞系 |
倍增时间 |
~18 .4-20 hours |
生长方式 |
贴壁 |
培养基和添加剂 |
DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%FBS(血清灭活)(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
ZM0092 |
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-1772 BCRC; 60083 BCRJ; 0058 CCTCC; GDC0175 CLS; 400476 DSMZ; ACC-565 ECACC; 91031101 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0092 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8544334/
2.论文标题: Programmed cell death 5 improves skeletal muscle insulin resistance by inhibiting IRS-1 ubiquitination through stabilization of MDM2
DOI: 10.1016/j.lfs.2021.119918
发表时间: 2021-09-01
期刊: LIFE SCIENCES
影响因子: 5.037
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S002432052100905X
3.论文标题: Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro
DOI: 10.1002/mnfr.201600673
发表时间: 2017-04-21
期刊: MOLECULAR NUTRITION & FOOD RESEARCH
影响因子: 4.323
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.201600673
4.论文标题: Identification of mangiferin as a potential Glucokinase activator by structure-based virtual ligand screening
DOI: 10.1038/srep44681
发表时间: 2017-03-20
期刊: Scientific Reports
影响因子: 4.259
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.nature.com/articles/srep44681
5.论文标题: Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis
DOI: 10.1016/j.yexcr.2020.111890
发表时间: 2020-02-05
期刊: EXPERIMENTAL CELL RESEARCH
影响因子: 3.905
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0014482720300896
6.论文标题: Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux
DOI: 10.1016/j.lfs.2020.118243
发表时间: 2020-08-10
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520309954
7.论文标题: Impaired autophagic flux contributes to muscle atrophy in obesity by affecting muscle degradation and regeneration
DOI: 10.1016/j.bbrc.2020.02.110
发表时间: 2020-02-24
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 2.985
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0006291X20303843
8.论文标题: Effects of acute hypoxia exposure with different durations on activation of Nrf2-ARE pathway in mouse skeletal muscle
DOI: 10.1371/journal.pone.0208474
发表时间: 2018-12-04
期刊: PLoS One
影响因子: 2.766
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208474
9.论文标题: Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes
DOI: 10.1016/j.acthis.2019.04.001
发表时间: 2019-04-30
期刊: ACTA HISTOCHEMICA
影响因子: 2.479
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0065128118304355
10.PubMed=563524; DOI=10.1038/270725a0
Yaffe D., Saxel O.
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
Nature 270:725-727(1977)
11.PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795
McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z., Oakeley A.E., Malouf N.N.
C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
Am. J. Physiol. 266:C1795-C1802(1994)
12.PubMed=15596414
Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F., Falcieri E.
C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
Eur. J. Histochem. 48:223-233(2004)
13.PubMed=19298647; DOI=10.1186/1471-2121-10-20
Herrera B., Inman G.J.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
BMC Cell Biol. 10:20.1-20.11(2009)
14.PubMed=19350625; DOI=10.1002/bit.22318
Fujita H., Shimizu K., Nagamori E.
Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
Biotechnol. Bioeng. 103:1034-1041(2009)
15.PubMed=20635352; DOI=10.1002/bit.22865
Fujita H., Endo A., Shimizu K., Nagamori E.
Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
Biotechnol. Bioeng. 107:894-901(2010)
16.PubMed=22020321; DOI=10.1016/j.yexcr.2011.10.004
Humphrey G.W., Mekhedov E., Blank P.S., de Morree A., Pekkurnaz G., Nagaraju K., Zimmerberg J.
GREG cells, a dysferlin-deficient myogenic mouse cell line.
Exp. Cell Res. 318:127-135(2012)
17.PubMed=24735950; DOI=10.1016/j.scr.2014.03.004
Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
Stem Cell Res. 12:742-753(2014)
18.PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)
19.PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0099 | So18小鼠骨骼肌成肌细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1700.00 | 放入购物车 》 |
ZQ0140 | L6大鼠成肌细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
5 | 荧光素酶标记 | ¥询价 | 放入购物车 》 | |
ZQ-200 | RPMI-1640基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-201 | RPMI-1640完全培养基 | 500ml | ¥350.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |
JSFW-134 | 人源细胞STR鉴定服务 | ¥900.00 | 放入购物车 》 |