毛囊外壳环绕毛发纤维周围或在根鞘内部。它与其它上皮成分不同，与表皮连接。毛囊外壳由具有独特的结构特性的几层细胞组成，对毛囊起着重要的作用，例如做为感应器官和皮肤的免疫防卫。免疫组织化学染色表明毛囊外壳包含嵌入式表达前体或干细胞。人类外根鞘细胞表现为重叠的角化细胞，在体外易于广泛培养。    

       人毛囊外根鞘细胞(HHORSC)提取于人毛囊外根鞘细胞，原代冻存。每管含有细胞数>5×105cells/ml，此细胞通过Fibronectin and CD105免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到10倍增。

推荐培养基:(MSCM, Cat. No. 7501)

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question:

       have a very different origin and function in the hair follicle. I want to buy a cell line that represents this. If you say your cell line stains for mesenchymal markers, your characterization of the cell line tells me that probably it is not a pure cell line and that you must certainly have a mixture of ORS cells and mesenchymal cells, like dermal sheath cells, since these mesenchymal cells surround ORS cells in the hair follicle That assumption is also present when you say the optimal medium is a mesenchymal stem cell medium. Most certainly a mesenchymal stem cell medium is not the optimal medium to culture epithelial cells. I need a pure cell line of ORS cell to compare to another epithelial population I have, otherwise I would be spending money in a wrong comparison because I would be using a cell line with a heterogeneous population of epithelial and mesenchymal cells. Please is there anyone that can give a clear answer to these questions? Someone directly involved in the isolation process of this cell line? It would be really important. I only have until next Tuesday to find and buy the cell line I need because after that the University financial department will prevent me from doing orders until next February. I would really appreciate your help. Best regards, Carla Abreu

Answers: