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人脑微血管内皮细胞
英文名:Human Brain Microvascular Endothelial Cells
货号:1000
价格:¥13362.00
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人脑微血管内皮细胞描述:


       人脑微血管内皮细胞是血脑屏障的主要组成成分,能够限制可溶性物质和细胞等从血液进入大脑。大脑微血管内皮细胞与外周内皮细胞相比具有一些相同特性。如:1)脑微血管内皮细胞存在许多细胞间紧密连接,产生很高的跨内皮阻抗,延迟细胞旁的通量;2)脑微血管内皮细胞缺乏内皮细胞的窗孔结构,其液相物质胞饮水平较低;3)脑微血管内皮细胞具有不对称定位酶和载体介导转运系统,从而产生 “两极分化”的表现型。 与外周内皮细胞相同,大脑微血管内皮细胞表面表达细胞粘附分子,调控白细胞进入大脑。由于微血管内皮细胞的器官特异性,内皮细胞通常取源于疾病研究的相关组织。 


      人脑微血管内皮细胞(HBMEC)提取于人脑组织,提取纯化之后立即冻存。每管含有细胞数 >5×105 cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。 

配套培养基:内皮细胞专用全能培养基ECM(NO.1001 


产品使用说明:仅供科研研究使用。

货号 1000
产地 美国
缩写 HBMEC
规格 5 x 10^5 cells/vial
用途 科研
运输 干冰
保存 液氮


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Question:
Which antibody was used to isolate the endothelial cells? Anti-CD31? Thanks


Answers:

Posted by Karishma Shah on Tuesday, April 12, 2016
Dear customer,
Yes, it is CD31.
1 Answer
I plan to use this product for in vitro blood brain barrier. So I have a few questions before I purchase this product 1. How many passages the cells can go through without loss of original characteristics 2. Can I prepare frozen stocks with these cells 3. What is the reported TEER with ScienCell HBMECs.
Posted by Pooja Dua , Thursday, January 7, 2016 on product Human Brain Microvascular Endothelial Cells
Question:

I plan to use this product for in vitro blood brain barrier. So I have a few questions before I purchase this product 1. How many passages the cells can go through without loss of original characteristics 2. Can I prepare frozen stocks with these cells 3. What is the reported TEER with ScienCell HBMECs.
Answers:

Posted by Manoj Sharma on Thursday, January 7, 2016
Hello Pooja,
It is highly recommended to use these cells at earliest passage right after revival without passaging. We do not recommend that customers re-freeze them for future use since it would damage the cells. In addition, #1000 HBMEC are out of stock.
thank you for your interest in our products.
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