名称 |
MDCK(NBL-2)狗肾细胞(种属鉴定) |
货号 |
ZQ0166 |
产品介绍 |
1958年九月 S.H. Madin and N.B. Darby从一只外观正常的成年雌性英国小猎犬的肾分离等到MDCK细胞株。角蛋白免疫过氧化物酶染色呈阳性。MDCK被用于研究β-粥样蛋白前体的处理及其蛋白水解产物。
注意事项: 我司提供MDCK细胞无血清专用培养基,欢迎咨询订购。 |
种属 |
狗 |
组织来源 |
原位;肾 |
形态学 |
上皮 |
细胞类型 |
自发永生化细胞系 |
倍增时间 |
|
生长方式 |
贴壁 |
培养基和添加剂 |
MEM(中乔新舟 货号:ZQ-300)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
AddexBio; P0014003/4928 ATCC; CCL-34 BCRC; 60004 BCRJ; 0168 BEI_Resources; NR-2628 CCLV; CCLV-RIE 0083 CCTCC; GDC0012 CCTCC; GDC0401 CLS; 602280 ECACC; 84121903 ECACC; 85011435 IBRC; C10556 IZSLER; BS CL 64 JCRB; IFO50071 JCRB; JCRB9029 KCB; KCB 2006105YJ KCLB; 10034 NCBI_Iran; C426 RCB; RCB0995 |
供应限制 |
仅供科研使用 |
货号 |
ZM0166 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=13901412; DOI=10.1126/science.138.3536.42
Green I.J.
Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells.
Science 138:42-43(1962)
PubMed=5918973; DOI=10.3181/00379727-122-31293
Gaush C.R., Hard W.L., Smith T.F.
Characterization of an established line of canine kidney cells (MDCK).
Proc. Soc. Exp. Biol. Med. 122:931-935(1966)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=222773; DOI=10.1083/jcb.81.3.635
Rindler M.J., Chuman L.M., Shaffer L., Saier M.H. Jr.
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
J. Cell Biol. 81:635-648(1979)
PubMed=6480288
Schroyens W., Bruyneel R., Tchao R., Leighton J., Dragonetti C.H., Mareel M.M.
Comparison of invasiveness and non-invasiveness of two epithelial cell lines in vitro.
Invasion Metastasis 4:160-170(1984)
PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8
Romanova J., Katinger D., Ferko B., Voglauer R., Mochalova L., Bovin N., Lim W., Katinger H., Egorov A.
Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=21819694
Omeir R.L., Teferedegne B., Foseh G.S., Beren J.J., Snoy P.J., Brinster L.R., Cook J.L., Peden K., Lewis A.M. Jr.
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells.
Comp. Med. 61:243-250(2011)
PubMed=21982418; DOI=10.1186/1471-2121-12-43
Dukes J.D., Whitley P., Chalmers A.D.
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43.1-43.4(2011)
PubMed=24058646; DOI=10.1371/journal.pone.0075014
Lugovtsev V.Y., Melnyk D., Weir J.P.
Heterogeneity of the MDCK cell line and its applicability for influenza virus research.
PLoS ONE 8:E75014-E75014(2013)
PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)
PubMed=32854295; DOI=10.3390/ijms21176111
Rodrigues A.F., Fernandes P., Laske T., Castro R., Alves P.M., Genzel Y., Coroadinha A.S.
Cell bank origin of MDCK parental cells shapes adaptation to serum-free suspension culture and canine adenoviral vector production.
Int. J. Mol. Sci. 21:6111.1-6111.27(2020)
PubMed=33122286; DOI=10.1681/ASN.2020010009
Khundmiri S.J., Chen L.-H., Lederer E.D., Yang C.-R., Knepper M.A.
Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
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