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MDCK(NBL-2)狗肾细胞(种属鉴定)

英文名:MDCK(NBL-2)
货号:ZQ0166
价格:¥1600.00
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推荐组合

MDCK(NBL-2)狗肾细胞(种属鉴定)

¥1600.00
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MDCK [NBL-2]狗肾细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥1950 ¥2080.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

MDCK(NBL-2)狗肾细胞(种属鉴定)

货号

ZQ0166

产品介绍

1958年九月 S.H. Madin and N.B. Darby从一只外观正常的成年雌性英国小猎犬的肾分离等到MDCK细胞株。角蛋白免疫过氧化物酶染色呈阳性。MDCK被用于研究β-粥样蛋白前体的处理及其蛋白水解产物。

注意事项:
MDCK细胞特别难消化,胰酶消化后细胞变圆脱落可冲散时,为消化完全。

我司提供MDCK细胞无血清专用培养基,欢迎咨询订购。

种属

组织来源

原位;肾

形态学

上皮

细胞类型

自发永生化细胞系

倍增时间

 

生长方式

贴壁

培养基和添加剂

MEM(中乔新舟  货号:ZQ-300+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%双抗(中乔新舟   货号:CSP006

推荐完全培养基货号

ZM0166

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37

保藏机构

AddexBio; P0014003/4928

ATCC; CCL-34

BCRC; 60004

BCRJ; 0168

BEI_Resources; NR-2628

CCLV; CCLV-RIE 0083

CCTCC; GDC0012

CCTCC; GDC0401

CLS; 602280

ECACC; 84121903

ECACC; 85011435

IBRC; C10556

IZSLER; BS CL 64

JCRB; IFO50071

JCRB; JCRB9029

KCB; KCB 2006105YJ

KCLB; 10034

NCBI_Iran; C426

RCB; RCB0995

供应限制

仅供科研使用


货号

ZM0166

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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Green I.J.
Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells.
Science 138:42-43(1962)

PubMed=5918973; DOI=10.3181/00379727-122-31293
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Characterization of an established line of canine kidney cells (MDCK).
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Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
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Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=21819694
Omeir R.L., Teferedegne B., Foseh G.S., Beren J.J., Snoy P.J., Brinster L.R., Cook J.L., Peden K., Lewis A.M. Jr.
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells.
Comp. Med. 61:243-250(2011)

PubMed=21982418; DOI=10.1186/1471-2121-12-43
Dukes J.D., Whitley P., Chalmers A.D.
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43.1-43.4(2011)

PubMed=24058646; DOI=10.1371/journal.pone.0075014
Lugovtsev V.Y., Melnyk D., Weir J.P.
Heterogeneity of the MDCK cell line and its applicability for influenza virus research.
PLoS ONE 8:E75014-E75014(2013)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=32854295; DOI=10.3390/ijms21176111
Rodrigues A.F., Fernandes P., Laske T., Castro R., Alves P.M., Genzel Y., Coroadinha A.S.
Cell bank origin of MDCK parental cells shapes adaptation to serum-free suspension culture and canine adenoviral vector production.
Int. J. Mol. Sci. 21:6111.1-6111.27(2020)

PubMed=33122286; DOI=10.1681/ASN.2020010009
Khundmiri S.J., Chen L.-H., Lederer E.D., Yang C.-R., Knepper M.A.
Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

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