人气管平滑肌细胞说明：
气道平滑肌是维持气道张力的根本。它存在于气管、支气管树直至终末支气管。其特异的超微结构特点和调节机制是实现气管正常生理功能的基础。有证据表明气管平滑肌在肺的发育过程以及诸如哮喘病、慢性支气管炎和肺气肿等疾病状态下经历着显著的表型变化。另外，气道平滑肌通过分泌细胞因子和趋化因子等促炎介质，发挥免疫调节功能。培养的气管平滑肌细胞能表达三种P21活化的激酶（PAK）的同型异构体和PAK1，并通过P38 mapk信号途径参与气管平滑肌细胞的迁移。气道平滑肌细胞增殖已成为人们关注的焦点，因为在哮喘病人中，此细胞是气道壁重塑反应中数量上的重要组分，并被认为是新型抗哮喘病药物的作用目标。
人气管平滑肌细胞(HTSMC)提取于人气管组织，原代冻存。每管含有细胞数>5×105cells/ml，此细胞通过对alpha-SMA和Desmin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基: (SMCM, Cat. No. 1101)
产品使用说明：仅供科研研究使用。
|
货号
|
3410
|
|
产地
|
美国
|
|
缩写
|
HTSMC
|
|
规格
|
5 x 10^5 cells/vial
|
|
用途
|
科研
|
|
运输
|
干冰
|
|
保存
|
液氮
|
1.) Timm DM, Chen J, Sing D, Gage JA, Haisler WL, Neeley SK, Raphael RM, Dehghani M, Rosenblatt KP, Killian TC, Tseng H, Souza GR. (2013) 'A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis.'
2.) Timm DM, Chen J, Sing D, Gage JA, Haisler WL, Neeley SK, Raphael RM, Dehghani M, Rosenblatt KP, Killian TC, Tseng H, Souza GR. (2013) "A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis." Sci rep. 3.
3.) Cheng SE, Lee IT, Lin CC, Kou YR, Yang CM. (2010) "Cigarette smoke particle-phase extract induces HO-1 expression in human tracheal smooth muscle cells: role of the c-Src/NADPH oxidase/MAPK/Nrf2 signaling pathway." Free Radic Biol Med. 48: 1410-22.
4.) Lee CW, Lee IT, Lin CC, Lee HC, Lin WN, Yang CM. (2010) "Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB." J Cell Biochem. 109: 1045-56.
5.) Cheng SE, Luo SF, Jou MJ, Lin CC, Kou YR, Lee IT, Hsieh HL, Yang CM. (2009) "Cigarette smoke extract induces cytosolic phospholipase A2 expression via NADPH oxidase, MAPKs, AP-1, and NF-kappaB in human tracheal smooth muscle cells." Free Radic Biol Med. 46: 948-60.
6.) Lin WN, Luo SF, Lin CC, Hsiao LD, Yang CM. (2009) "Differential involvement of PKC-dependent MAPKs activation in lipopolysaccharide-induced AP-1 expression in human tracheal smooth muscle cells." Cell Signal. 21: 1385-95.
7.) Luo SF, Chang CC, Lee IT, Lee CW, Lin WN, Lin CC, Yang CM. (2009) "Activation of ROS/NF-kappaB and Ca2+/CaM kinase II are necessary for VCAM-1 induction in IL-1beta-treated human tracheal smooth muscle cells." Toxicol Appl Pharmacol. 237: 8-21.
8.) Yang CM, Lee IT, Lin CC, Yang YL, Luo SF, Kou YR, Hsiao LD. (2009) "Cigarette smoke extract induces COX-2 expression via a PKCalpha/c-Src/EGFR, PDGFR/PI3K/Akt/NF-kappaB pathway and p300 in tracheal smooth muscle cells." Am J Physiol Lung Cell Mol Physiol. 297: L892-902.
9.) Lee CW, Lin CC, Luo SF, Lee HC, Lee IT, Aird WC, Hwang TL, Yang CM. (2008) "Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells." Mol Pharmacol. 73: 1454-64.
10.) Liang KC, Lee CW, Lin WN, Lin CC, Wu CB, Luo SF, Yang CM. (2007) "Interleukin-1beta induces MMP-9 expression via p42/p44 MAPK, p38 MAPK, JNK, and nuclear factor-kappaB signaling pathways in human tracheal smooth muscle cells." J Cell Physiol. 211: 759-70.
11.) Song Y, Qi H, Wu C. (2007) "Effect of 1,25-(OH)2D3 (a vitamin D analogue) on passively sensitized human airway smooth muscle cells." Respirology. 12: 486-94.
There are no product questions yet.
上海中乔新舟生物科技有限公司
微信扫一扫咨询
