上海中乔新舟生物科技有限公司

ScienCell's GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:

Cell cycle regulation: CCNT1, CCNT2, CDK9, CDK14, CDKN2C
Signaling mediation: IGF1R, BMP2K, PROM1
Differentiation regulation: CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
Mutated transcription factors identified

in acute myeloid leukemia: AML1, CEBPA, PLZF, ZBTB16, SPI1
in precursor B lymphoblastic leukemia: TCF3, PBX1
in precursor T lymphoblastic leukemia: TLX1, TLX3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Plate Layout*(8 controls in Bold and Italic)

AFF1

CCND2

CDKN1A

DUSP6

HDAC1

HPGD

KLF3

MEIS1

PBX1

RUNX2

TCF3

β-actin

ATM

CCNT1

CDKN2C

ETV6

HDAC2

IGF1

KMT2A

MLLT1

PER2

SIN3A

TLX1

GAPDH

BCL2A1

CCNT2

CEBPA

ETV7

HHEX

IGF1R

LDB1

MLLT3

PROM1

SIX1

TLX3

LDHA

BCL2L1

CCR7

CSF1R

EYA1

HIST2H3C

IL3

LMO2

MYC

PTCRA

SIX4

TRAF1

NONO

BCL6

CD14

CSF2

FCGR1A

HMGA2

ITGB7

LYL1

MYCN

RARA

SMAD1

UTY

PPIH

BIRC3

CD86

DDIT3

FUT8

HOXA10

JMJD1C

MAF

NCOR1

REL

SP1

WHSC1

GDC

BMP2K

CDK14

DEFA3

GRIA3

HOXA11

JUP

MAX

NFKB1

RUNX1

SPI1

WNT16

PPC

CCNA1

CDK9

DOT1L

GZMB

HOXA9

KDM6A

MEF2C

PAX5

RUNX1T1

SUPT3H

ZBTB16

NTC

* gene selection may be updated based on new research and development

产品使用说明:仅供科研研究使用

货号	GK030
产地	美国
缩写	GQH-TMH
规格	One 96-well plate, one 20ul reaction per well
用途	科研
保存	4°C or -20°C
运输	常温

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公司简介 / Company profile

上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
上海中乔新舟生物科技有限公司（官网：www.zqxzbio.com）成立于2011年，历经十多年发展，...

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