人黑素细胞发育与色素沉着定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ human melanocyte development & pigmentation qPCR array kit (GQH-MDP) is designed to facilitate gene expression profiling of key genes involved in melanocyte development and pigmentation pathways. 40 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Melanocyte Development & Pigmentation qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ADCY3 |
CTNNB1 |
GRB2 |
MC1R |
PRKACA |
β-actin |
ADCY3 |
CTNNB1 |
GRB2 |
MC1R |
PRKACA |
β-actin |
|
B |
AKT1 |
EDNRB |
GSK3B |
MITF |
QPCT |
GAPDH |
AKT1 |
EDNRB |
GSK3B |
MITF |
QPCT |
GAPDH |
|
C |
AKT3 |
EP300 |
HRAS |
MLANA |
RPS6KA1 |
LDHA |
AKT3 |
EP300 |
HRAS |
MLANA |
RPS6KA1 |
LDHA |
|
D |
BCL2 |
FOXD3 |
KIT |
OCA2 |
SOS1 |
NONO |
BCL2 |
FOXD3 |
KIT |
OCA2 |
SOS1 |
NONO |
|
E |
BRAF |
FZD3 |
KITLG |
PAX3 |
SOX10 |
PPIH |
BRAF |
FZD3 |
KITLG |
PAX3 |
SOX10 |
PPIH |
|
F |
CDH1 |
FZD4 |
LEF1 |
PIK3CA |
TRP2 |
GDC |
CDH1 |
FZD4 |
LEF1 |
PIK3CA |
TRP2 |
GDC |
|
G |
CREB1 |
GNB2L1 |
MAPK1 |
PMEL |
TYR |
PPC |
CREB1 |
GNB2L1 |
MAPK1 |
PMEL |
TYR |
PPC |
|
H |
CREBBP |
GPR143 |
MAPK3 |
POMC |
TYRP1 |
NTC |
CREBBP |
GPR143 |
MAPK3 |
POMC |
TYRP1 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK010 |
|
产地 |
美国 |
|
缩写 |
GQH-MDP |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 |
| 2200 | 人表皮黑色素细胞-浅色素 | 5 x 10^5 cells/vial | ￥9756.00 | 放入购物车 》 |
| 2210 | 人表皮黑色素细胞--中色素 | 5 x 10^5 cells/vial | ￥9990.00 | 放入购物车 》 |
| 2220 | 人表皮黑色素细胞--深色素 | 5 x 10^5 cells/vial | ￥9990.00 | 放入购物车 》 |
| 2230 | 人黑色素细胞--成人 | 5 x 10^5 cells/vial | ￥9504.00 | 放入购物车 》 |
| 2201 | 黑色素细胞培养基 | 500ML | ￥2176.00 | 放入购物车 》 |
| 2211 | 黑色素细胞培养基-2 | 500ML | ￥2176.00 | 放入购物车 》 |
| 2204 | 人表皮黑素细胞-浅色素cDNA | 20 reactions | ￥6552.00 | 放入购物车 》 |
| 2214 | 人表皮黑素细胞-中色素cDNA | 20 reactions | ￥6552.00 | 放入购物车 》 |
| 2224 | 人表皮黑素细胞-深色素cDNA | 20 reactions | ￥6552.00 | 放入购物车 》 |
| 2234 | 人表皮黑素细胞-成人的cDNA | 20 reactions | ￥6552.00 | 放入购物车 》 |
| 2205 | 人表皮黑素细胞-浅色素总RNA | 10 μg | ￥5814.00 | 放入购物车 》 |
| 2215 | 人表皮黑素细胞-中色素总RNA | 10 μg | ￥5814.00 | 放入购物车 》 |
| 2225 | 人表皮黑素细胞-深色素总RNA | 10 μg | ￥5814.00 | 放入购物车 》 |
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