您是第 11157408 位 欢迎访问 中乔新舟官网 ! 全国免费电话:400-038-9959 我的购物车(0) 注 册 / 登 录
扫码关注公众号
您当前的位置:首页 > 产品中心

SK-MEL-1人皮肤黑色素瘤细胞(STR鉴定)

英文名:SK-MEL-1
货号:ZQ0835
价格:¥1350.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
推荐组合

SK-MEL-1人皮肤黑色素瘤细胞(STR鉴定)

¥1350.00
+

SK-MEL-1人皮肤黑色素瘤细胞专用培养基

¥350.00 ¥450.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1700 ¥1950.00
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

SK-MEL-1人皮肤黑色素瘤细胞

货号

ZQ0835

产品介绍

该细胞由OettgenF及其同事从一名29岁的患有广泛、快速进展性恶性黑色素瘤的白人男性患者的胸导管中分离建立的。该细胞可产生黑色素,电镜检测发现细胞中色素颗粒与自身合成和吞噬作用相关。在63%的恶性黑色素瘤患者和10%其他疾病患者体内发现了针对该细胞系的抗体。根据相关机构的测序、WB 和 PCR 结果,该细胞系携带 BRAF V600E 突变。细胞是 N-Ras 野生型。 

种属

性别/年龄

男/29岁

组织

皮肤

疾病

黑素瘤

细胞类型

黑素细胞

形态学

球形

生长方式

悬浮

倍增时间

大约100小时 

培养基和添加剂

MEM(含NEAA)培养基(中乔新舟  货号:ZQ-300) +10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0835

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 12
TH01: 6
TPOX: 11
vWA: 16,17
D3S1358: 14,16
D21S11: 29,32.2
D18S51: 13,16
Penta_E: 7,21
Penta_D: 11,13
D8S1179: 13,16
FGA: 18,20
D19S433: 15,16.2
D2S1338: 19,23

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; HTB-67 DSMZ; ACC-303 KCLB; 30067

供应限制

仅供科研使用

货号

ZQ0835

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


论文标题: Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN
DOI: 10.3892/ijo.2018.4414
发表时间: 2018-08-01
期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
影响因子: 5.65
货号: ZQ0835
产品名称: SK-MEL-1 cells

原文链接: https://www.spandidos-publications.com/10.3892/ijo.2018.4414?text=abstract



PubMed=4879578; DOI=10.1093/jnci/41.4.827
Oettgen H.F., Aoki T., Old L.J., Boyse E.A., de Harven E., Mills G.M.
Suspension culture of a pigment-producing cell line derived from a human malignant melanoma.
J. Natl. Cancer Inst. 41:827-843(1968)


PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)


PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)


PubMed=6895502; DOI=10.1002/eji.1830111015
Johnson J.P., Demmer-Dieckmann M., Meo T., Hadam M.R., Riethmuller G.
Surface antigens of human melanoma cells defined by monoclonal antibodies. I. Biochemical characterization of two antigens found on cell lines and fresh tumors of diverse tissue origin.
Eur. J. Immunol. 11:825-831(1981)


PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)


PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)


PubMed=3335022
Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)


PubMed=8755562; DOI=10.1073/pnas.93.15.7832
Liu D.-C., Pearlman E., Diaconu E., Guo K., Mori H., Haqqi T., Markowitz S.D., Willson J.K.V., Sy M.-S.
Expression of hyaluronidase by tumor cells induces angiogenesis in vivo.
Proc. Natl. Acad. Sci. U.S.A. 93:7832-7837(1996)


PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)


PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)


PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)


PubMed=21725359; DOI=10.1038/onc.2011.250
Xing F., Persaud Y., Pratilas C.A., Taylor B.S., Janakiraman M., She Q.-B., Gallardo H., Liu C., Merghoub T., Hefter B., Dolgalev I., Viale A.J., Heguy A., de Stanchina E., Cobrinik D., Bollag G., Wolchok J.D., Houghton A.N., Solit D.B.
Concurrent loss of the PTEN and RB1 tumor suppressors attenuates RAF dependence in melanomas harboring (V600E)BRAF.
Oncogene 31:446-457(2012)


PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)


公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
      上海中乔新舟生物科技有限公司(官网:www.zqxzbio.com)成立于2011年,历经十多年发展,...
联系我们 / Contact
电 话:021-56760357;021-56760351
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved. 网站地图

沪公网安备 31011002001038号

技术支持:攸攸网络 沪ICP备14008091号