产品名称 |
M-14人黑色素瘤细胞 |
货号 |
ZQ0045 |
产品介绍 |
M-14(也称为M14、UCLA-SO-M14、Melanoma14或M-14)是一种人黑色素瘤细胞系,最初是从一位33岁男性患者的无色素性黑色素瘤中分离建立的。这种细胞系在黑色素瘤的研究中具有重要价值,尤其是在药物筛选和肿瘤生物学特性研究方面。是黑色素瘤研究领域中的一个重要工具,它为科学家们提供了一个模拟人类黑色素瘤在体外条件下行为的模型。 |
种属 |
人 |
性别/年龄 |
男/33岁 |
组织 |
转移性;右臀,皮下组织 |
疾病 |
黑素瘤 |
细胞类型 |
肿瘤细胞 |
形态学 |
淋巴母细胞样 |
生长方式 |
半贴半悬 |
倍增时间 |
~26.3小时 |
培养基和添加剂 |
DMEM(高糖)基础培养基(中乔新舟 货号:ZQ-100)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%PS(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL - 1 |
STR位点信息 |
Amelogenin:X; |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
CLS;302163 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0045 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
1、PubMed=56444; DOI=10.1093/jnci/56.1.167
Golub S.H., Hanson D.C., Sulit H.L., Morton D.L., Pellegrino M.A., Ferrone S.
Comparison of histocompatibility antigens on cultured human tumor cells and fibroblasts by quantitative antibody absorption and sensitivity to cell-mediated cytotoxicity.
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Zielske J.V., Golub S.H.
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Human tumor cells grown in fetal calf serum and human serum: influences on the tests for lymphocyte cytotoxicity, serum blocking and serum arming effects.
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Chee D.O., Boddie A.W. Jr., Roth J.A., Holmes E.C., Morton D.L.
Production of melanoma-associated antigen(s) by a defined malignant melanoma cell strain grown in chemically defined medium.
Cancer Res. 36:1503-1509(1976)
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Saxton R.E., Irie R.F., Ferrone S., Pellegrino M.A., Morton D.L.
Establishment of paired tumor cells and autologous virus-transformed cell lines to define humoral immune responses in melanoma and sarcoma patients.
Int. J. Cancer 21:299-306(1978)
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Chee D.O., Gupta R.K., Morton D.L.
Humoral response of melanoma patients to two different tumor-associated antigens.
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Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice.
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Heat-shock proteins produced by two human melanoma cell lines: absence of correlation with thermosensitivity.
Int. J. Cancer 34:651-655(1984)
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Takahashi H., Adachi S., Yoshiya N., Suzuki T., Kanazawa K., Takeuchi S.
Expression of HLA-DR molecules in human gestational choriocarcinoma cell lines and malignant cell lines.
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Greco C., Zupi G.
Biological features and in vitro chemosensitivity of a new model of human melanoma.
Anticancer Res. 7:839-844(1987)
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Wong J.H., Aguero B., Gupta R.K., Morton D.L.
Recovery of a cell surface fetal antigen from circulating immune complexes of melanoma patients.
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Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase-type plasminogen activator, its type-1 inhibitor, and urokinase-mediated matrix degradation.
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Systematic variation in gene expression patterns in human cancer cell lines.
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Human melanoma cells secrete and respond to placenta growth factor and vascular endothelial growth factor.
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Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)
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HLA class I and II genotype of the NCI-60 cell lines.
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Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
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Systems-level modeling of cancer-fibroblast interaction.
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28、PubMed=22068913; DOI=10.1073/pnas.1111840108
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29、PubMed=22347499; DOI=10.1371/journal.pone.0031628
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A landscape of pharmacogenomic interactions in cancer.
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A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
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Characterization of human cancer cell lines by reverse-phase protein arrays.
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Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.
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Genetic alterations in main candidate genes during melanoma progression.
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42、PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
43、PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0042 | A375人恶性黑色素瘤细胞(STR鉴定)[套餐促销] | 5 x 10^5 cells/vial | ¥1200.00 | 放入购物车 》 |
ZQ0108 | B16-F10小鼠黑色素瘤高转细胞(str鉴定)[套餐促销] | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0186 | B16小鼠黑色素瘤细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1000.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
7 | 细胞基因敲除 | ¥询价 | 放入购物车 》 | |
ZQ-100 | DMEM(高糖)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-101 | DMEM(高糖)完全培养基 | 500ML | ¥350.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |
JSFW-134 | 人源细胞STR鉴定服务 | ¥900.00 | 放入购物车 》 | |
GK012 | 人黑色素瘤定量PCR芯片试剂盒 | One 96-well plate, one 20ul reaction per well | ¥4320.00 | 放入购物车 》 |