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MM.1S人IgA-骨髓瘤细胞 (STR鉴定)

英文名:MM.1S
货号:ZQ0485
价格:¥1500.00
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推荐组合

MM.1S人IgA-骨髓瘤细胞 (STR鉴定)

¥1500.00
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MM.1S人IgA-骨髓瘤细胞专用培养基

¥550.00

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细胞套餐惊爆价

¥2050
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MM.1S人IgA-骨髓瘤细胞

货号

ZQ0485

产品介绍

MM.1S是一种人IgA-骨髓瘤细胞系,最初是从患有免疫球蛋白Aλ骨髓瘤的42岁黑人女性患者的外周血中分离的B淋巴母细胞建立的,这些细胞具有淋巴母细胞样的形态,并且既可以悬浮生长也可以轻微贴壁。可用于研究多发性骨髓瘤的疾病进展、药物治疗反应以及耐药性机制。对地塞米松敏感。

注意事项:
(1)该细胞同时存在悬浮生长和轻微贴壁状态。
(2)如果培养过程中培养液逐渐变黄,可加入少量新鲜培养液或分瓶。
(3)如果贴壁部分的细胞融合度达到80%以上,需要用细胞刮将贴壁细胞刮下,和悬浮的细胞一并收集离心,1000rpm,5min,去掉上清液后,加入新鲜培养液重悬细胞并分瓶。
(4)贴壁细胞不能过度融合。
(5)该细胞复苏后需培养2周左右时间,才能恢复正常生长状态。

种属

性别/年龄

女/42岁

组织

外周血

疾病

免疫球蛋白Aλ骨髓瘤

细胞类型

B淋巴细胞

形态学

淋巴母细胞

生长方式

半悬浮

倍增时间

大约72~80小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%双抗(中乔新舟  货号:CSP006)+1% Sodium Pyruvate 100 mM Solution(中乔新舟  货号:CSP003) +1%L-alanyl-L-glutamine(中乔新舟   货号:CSP004

推荐完全培养基货号

ZM0485

生物安全等级

BSL-1

STR位点信息

Amelogenin: X
CSF1PO: 9,14
D13S317: 13
D16S539: 12
D5S818: 8,12
D7S820: 10,11
TH01: 7,8
TPOX: 8
vWA: 15,17
D3S1358: 16,17
D21S11: 29,30
D18S51: 11,16
Penta_E: 13,15
Penta_D: 2.2,9
D8S1179: 14
FGA: 20,24
D19S433: 13
D2S1338: 21,22

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

文献报道抗原表达CD25、CD38、CD52和CD59,糖皮质激素受体。 

基因表达

***  

保藏机构

ATCC; CRL-2974

供应限制

仅供科研使用

货号

ZQ0485

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


1、论文标题: Globular C1q Receptor (gC1qR/p32/HABP1) Suppresses the Tumor-Inhibiting Role of C1q and Promotes Tumor Proliferation in 1q21-Amplified Multiple Myeloma
DOI: 10.3389/fimmu.2020.01292
发表时间: 2020-07-14
期刊: Frontiers in Immunology
影响因子: 5.085
货号: ZQ0485
产品名称: MM1S cells

原文链接: https://www.frontiersin.org/articles/10.3389/fimmu.2020.01292/full



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PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
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PubMed=25485619; DOI=10.1038/nbt.3080
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CLPUB00604

Chow S.
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