产品名称 |
Y79人视网膜母细胞瘤细胞 |
货号 |
ZQ0904 |
产品介绍 |
这株y79系由t.w. reid及其同事于1971年1月通过摘除后立即获得的右眼原发肿瘤的外植体培养分离。捐赠者有很强的视网膜母细胞瘤家族史。据报道,超微结构特征包括核膜信息、三重膜结构、微管、大包膜泡、中心粒、基体和环状片层与原肿瘤相似。 注意事项:
Y79细胞悬浮聚团生长,细胞传代时需要冲散细胞为单细胞后传代。细胞复苏生长较慢,需要1周左右生长。 |
种属 |
人 |
性别/年龄 |
女/2.5岁 |
组织 |
眼睛,视网膜 |
疾病 |
视网膜母细胞瘤;视网膜神经胶质细胞瘤 |
细胞类型 |
肿瘤细胞 |
形态学 |
多细胞集群、呈葡萄状聚集 |
生长方式 |
悬浮 |
倍增时间 |
大约1周左右 |
培养基和添加剂 |
RPMI-1640(中乔新舟 货号:ZQ-200)+20%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X
CSF1PO: 11,12 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; HTB-18 BCRC; 60422 BCRJ; 0380 DSMZ; ACC-246 ECACC; 86093003 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0904 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925320/
PubMed=4135597; DOI=10.1093/jnci/53.2.347
Reid T.W., Albert D.M., Rabson A.S., Russell P., Craft J.L., Chu E.W., Tralka T.S., Wilcox J.L.
Characteristics of an established cell line of retinoblastoma.
J. Natl. Cancer Inst. 53:347-360(1974)
PubMed=679190
McFall R.C., Nagy R.M., Nagle B.T., McGreevy L.M.
Scanning electron microscopic observation of two retinoblastoma cell lines.
Cancer Res. 38:2827-2835(1978)
PubMed=6167750; DOI=10.1093/jnci/67.2.301
Gilbert F., Balaban G.B., Breg W.R., Gallie B.L., Reid T.W., Nichols W.W.
Homogeneously staining region in a retinoblastoma cell line: relevance to tumor initiation and progression.
J. Natl. Cancer Inst. 67:301-306(1981)
PubMed=6351622; DOI=10.1016/S0002-9394(14)77822-5
Ohashi Y., Sasabe T., Nishida T., Nishi Y., Higashi H.
Hanganutziu-Deicher heterophile antigen in human retinoblastoma cells.
Am. J. Ophthalmol. 96:321-325(1983)
DOI=10.5795/jjscc.24.451
Tsumuraya M., Nakajima T., Terasaki T., Kodama T., Shimosato Y., Higuchi H., Uei Y.
Analysis of LDH isoenzyme patterns in cell lines of the small cell carcinoma (lung) and retinoblastoma.
Nihon Rinsho Saibo Gakkai Zasshi 24:451-456(1985)
PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)
PubMed=3413073; DOI=10.1073/pnas.85.16.6017
Lee E.Y.-H.P., Bookstein R., Young L.-J., Lin C.-J., Rosenfeld M.G., Lee W.-H.
Molecular mechanism of retinoblastoma gene inactivation in retinoblastoma cell line Y79.
Proc. Natl. Acad. Sci. U.S.A. 85:6017-6021(1988)
PubMed=2917337; DOI=10.1016/0165-4608(89)90079-4
Horsthemke B., Greger V., Becher R., Passarge E.
Mechanism of i(6p) formation in retinoblastoma tumor cells.
Cancer Genet. Cytogenet. 37:95-102(1989)
PubMed=1679230; DOI=10.3109/13816819109023085
Madreperla S.A., Bookstein R., Jones O.W., Lee W.-H.
Retinoblastoma cell lines Y79, RB355 and WERI-Rb27 are genetically related.
Ophthalmic Paediatr. Genet. 12:49-56(1991)
PubMed=7689221; DOI=10.1073/pnas.90.16.7578
Godbout R., Squire J.A.
Amplification of a DEAD box protein gene in retinoblastoma cell lines.
Proc. Natl. Acad. Sci. U.S.A. 90:7578-7582(1993)
PubMed=7499444; DOI=10.1007/BF01213319
Inomata M., Saijo N., Kawashima K., Kaneko A., Fujiwara Y., Kunikane H., Tanaka Y.
Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid.
J. Cancer Res. Clin. Oncol. 121:729-738(1995)
PubMed=18036396; DOI=10.1016/j.cancergencyto.2007.08.014
Paderova J., Orlic-Milacic M., Yoshimoto M., da Cunha Santos G., Gallie B.L., Squire J.A.
Novel 6p rearrangements and recurrent translocation breakpoints in retinoblastoma cell lines identified by spectral karyotyping and mBAND analyses.
Cancer Genet. Cytogenet. 179:102-111(2007)
PubMed=18799932; DOI=10.1097/MPH.0b013e31816e232d
Kim J.H., Kim J.H., Yu Y.S., Kim D.H., Kim Y.K., Kim K.-W.
Comparative genomic hybridization analysis of newly established retinoblastoma cell lines of adherent growth compared with Y79 of nonadherent growth.
J. Pediatr. Hematol. Oncol. 30:571-574(2008)
PubMed=19686387; DOI=10.1111/j.1471-4159.2009.06322.x
Glubrecht D.D., Kim J.-H., Russell L., Bamforth J.S., Godbout R.
Differential CRX and OTX2 expression in human retina and retinoblastoma.
J. Neurochem. 111:250-263(2009)
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)
PubMed=21697133; DOI=10.1167/iovs.11-7479
Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.
Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011)
PubMed=23498719; DOI=10.1016/S1470-2045(13)70045-7
Rushlow D.E., Mol B.M., Kennett J.Y., Yee S., Pajovic S., Theriault B.L., Prigoda-Lee N.L., Spencer C., Dimaras H., Corson T.W., Pang R., Massey C., Godbout R., Jiang Z., Zacksenhaus E., Paton K., Moll A.C., Houdayer C., Raizis A., Halliday W., Lam W.L., Boutros P.C., Lohmann D.R., Dorsman J.C., Gallie B.L.
Characterisation of retinoblastomas without RB1 mutations: genomic, gene expression, and clinical studies.
Lancet Oncol. 14:327-334(2013)
货号 | 产品名称 | 规格 | 价格 | 指令 |