人星形胶质细胞描述：
在哺乳动物的大脑中，绝大部分细胞是星形胶质细胞。它们形态各异，并与神经元各个部分紧密相连，因此可能具有多种功能。星形胶质细胞为神经元提供结构，营养和代谢上的支持并调节突触活动。通过敲除或其它方式损坏星形胶质细胞可严重影响神经元的存活。此外，星形胶质细胞可提示许多神经疾病的病理过程。脑损伤后，通过神经突生长，突触可塑性和神经元再生的长期恢复过程受星形胶质细胞表面分子的表达和营养因子释放的影响。另外，星形胶质细胞自身存活或死亡可能影响最终的临床结果。星形胶质细胞在中枢神经系统功能的重要性越来越被重视，尤其是对于神经活动的调节。我们所获得的有关星形胶质细胞的知识主要来源于体外研究，星形胶质细胞的培养为探索细胞多样性提供了重要工具。
人星形胶质细胞(HA)提取于人脑(大脑皮层)，小神经胶质细胞(HAc)提取于人小脑组织。原代培养末期冻存，每管含有细胞数>5x106cells/ml，此细胞通过GFAP抗体荧光免疫方法验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到10-15倍增。
配套培养基：星形胶质细胞培养基（AM.NO.1801)
产品使用说明：仅供科研研究使用
1.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12
2.) Shang, C., Hong, Y., Guo, Y. & Xue, Y. (2016) Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene Med Sci Monit. 22
3.) Olsson, M., Hultman, K., Dunoyer-Geindre, S., Curtis, M.A., Faull, R.L.M., Kruithof, E.K.O. & Jern, C. (2016) Epigenetic Regulation of Tissue-Type Plasminogen Activator in Human Brain Tissue and Brain-Derived Cells Gene Regul Syst Bio. 10
4.) Liang, Y., Xie, X., Chen, L., Yan, S., Ye, X., Anjum, K., Huang, H., Lian, X. & Zhang, Z. (2016) Bioactive Polycyclic Quinones from Marine Streptomyces sp. 182SMLY Mar Drugs. 14
5.) Herland, A., van der Meer, A.D., FitzGerald, E.A., Park, T.E., Sleeboom, J.J.F. & Ingber, D.E. (2016) Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip PLoS One. 11
6.) Zhang, Y., Zhu, T., Zhang, X., Chao, J., Hu, G. & Yao, H. (2015) Role of high-mobility group box 1 in methamphetamine-induced activation and migration of astrocytes J Neuroinflammation. 12
7.) Verheyen, A., Diels, A., Dijkmans, J., Oyelami, T., Meneghello, G., Mertens, L., Versweyveld, S., Borgers, M., Buist, A., Peeters, P. & Cik, M. (2015) Using Human iPSC-Derived Neurons to Model TAU Aggregation PLoS One. 10
8.) Sreekanthreddy, P., Gromnicova, R., Davies, H., Phillips, J., Romero, I.A. & Male, D. (2015) A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions F1000Res. 4
9.) Rath, B.H., Wahba, A., Camphausen, K. & Tofilon, P.J. (2015) Coculture with astrocytes reduces the radiosensitivity of glioblastoma stem-like cells and identifies additional targets for radiosensitization Cancer Med. 4
10.) Pan, J., Wang, H., Liu, X., Hu, J., Song, W., Luo, J., Jiang, S., Yan, F. & Zhai, B. (2015) Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS PromoterPLoS One. 10
11.) Lin, J.J., Zhao, T.Z., Cai, W.K., Yang, Y.X., Sun, C., Zhang, Z., Xu, Y.Q., Chang, T. & Li, Z.Y. (2015) Inhibition of histamine receptor 3 suppresses glioblastoma tumor growth, invasion, and epithelial-to-mesenchymal transition Oncotarget. 6
12.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
13.) Kano, S., Yuan, M., Cardarelli, R.A., Maegawa, G., Higurashi, N., Gaval-Cruz, M., Wilson, A.M., Tristan, C., Kondo, M.A., Chen, Y., Koga, M., Obie, C., Ishizuka, K., Seshadri, S., Srivastava, R., Kato, T.A., Horiuchi, Y., Sedlak, T.W., Lee, Y., Rapoport, J.L., Hirose, S., Okano, H., Valle, D., O'Donnell, P., Sawa, A. & Kai, M. (2015) Clinical utility of neuronal cells directly converted from fibroblasts of patients for neuropsychiatric disorders: studies of lysosomal storage diseases and channelopathy Curr Mol Med. 15
14.) Jiang, L., Wang, C., Lei, F., Zhang, L., Zhang, X., Liu, A., Wu, G., Zhu, J. & Song, L. (2015) miR-93 Promotes Cell Proliferation in Gliomas through Activation of PI3K/Akt Signaling Pathway Oncotarget. 6
15.) Janmaat, C.J., de Rooij, K.E., Locher, H., de Groot, S.C., de Groot, J., Frijns, J.H.M. & Huisman, M.A. (2015) Human Dermal Fibroblasts Demonstrate Positive Immunostaining for Neuron- and Glia- Specific Proteins PLoS One. 10
16.) Hind, W.H., Tufarelli, C., Neophytou, M., Anderson, S.I., England, T.J. & O'Sullivan, S.E. (2015) Endocannabinoids modulate human blood?brain barrier permeability in vitro Br J Pharmacol. 172
17.) Garwood, C.J., Ratcliffe, L.E., Morgan, S.V., Simpson, J.E., Owens, H., Vazquez-Villase�or, I., Heath, P.R., Romero, I.A., Ince, P.G. & Wharton, S.B. (2015) Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors Mol Brain. 8
18.) David, C.N., Frias, E.S., Elix, C.C., McGovern, K.E., Walker, A.M., Eichler, J.F. & Wilson, E.H. (2015) Antitumor Activity of a Polypyridyl Chelating Ligand: In Vitro and In Vivo Inhibition of Glioma ASN Neuro. 7
19.) Zhou Y, Hayashi I, Wong J, Tugusheva K, Renger JJ, Zerbinatti C. (2014) 'Intracellular Clusterin Interacts with Brain Isoforms of the Bridging Integrator 1 and with the Microtubule-Associated Protein Tau in Alzheimer's Disease.'
20.) Monticone M, Taherian R, Stigliani S, Carra E, Monteghirfo S, Longo L, Daga A, Dono M, Giaretti W, Castagnola P. (2014) 'NAC, Tiron and Trolox Impair Survival of Cell Cultures Containing Glioblastoma Tumorigenic Initiating Cells by Inhibition of Cell Cycle Progression.'
21.) Cruz-Orengo L, Daniels BP, Dorsey D, Basak SA, Grajales-Reyes JG, McCandless EE, Piccio L, Schmidt RE, Cross AH, Crosby SD, Klein RS. (2014) 'Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.' J clin invest.
22.) Cruz-Orengo L, Daniels BP, Dorsey D, Basak SA, Grajales-Reyes JG, McCandless EE, Piccio L, Schmidt RE, Cross AH, Crosby SD, Klein RS. (2014) "Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility." J clin invest. 124: 2571-84.
23.) Monticone M, Taherian R, Stigliani S, Carra E, Monteghirfo S, Longo L, Daga A, Dono M, Giaretti W, Castagnola P. (2014) "NAC, Tiron and Trolox Impair Survival of Cell Cultures Containing Glioblastoma Tumorigenic Initiating Cells by Inhibition of Cell Cycle Progression." PLOS ONE. 9: e90085.
24.) Zhou Y, Hayashi I, Wong J, Tugusheva K, Renger JJ, Zerbinatti C. (2014) "Intracellular Clusterin Interacts with Brain Isoforms of the Bridging Integrator 1 and with the Microtubule-Associated Protein Tau in Alzheimer's Disease." PloS one. 9: e103187.
25.) Capozzi, M. E., McCollum, G. W., & Penn, J. S. (2014). The Role of Cytochrome P450 Epoxygenases in Retinal Angiogenesis. Investigative Ophthalmology & Visual Science, 55(7), 4253�4260. http://doi.org/10.1167/iovs.14-14216
26.) Urich E, Patsch C, Aigner S, Graf M, Iacone R, Freskgrd P. (2013) "Multicellular self-assembled spheroidal model of the blood brain barrier." Sci Rep. 3: 1500.
27.) Abdelmoaty S, Wigerblad G, Bas DB, Codeluppi S, Fernandez-Zafra T, El-Awady ES, Moustafa Y, Abdelhamid AE, Brodin E, Svensson CI. (2013) "Spinal Actions of Lipoxin A4 and 17(R)-Resolvin D1 Attenuate Inflammation-Induced Mechanical Hypersensitivity and Spinal TNF Release." PLoS One. 8: e75543.
28.) Cucullo L, Hossain M, Tierney W, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: Cerebrovascular physiology in a box." BMC Neuroscience. 14:18
29.) Vijayaraghavan S, Karami A, Aeinehband S, Behbahani H, Grandien A, Nilsson B, Ekdahl KN, Lindblom RP, Piehl F, Darreh-Shori T. (2013) "Regulated Extracellular Choline Acetyltransferase Activity - The Plausible Missing Link of the Distant Action of Acetylcholine in the Cholinergic Anti-Inflammatory Pathway." PLoS One. 8: e65936
30.) Li J, Bonifati S, Hristov G, Marttila T, Valmary Degano S, Stanzel S, Schnolzer M, Mougin C, Aprahamian M, Grekova SP, Raykov Z, Rommelaere J, Marchini A. (2013) 'Synergistic combination of valproic acid and oncolytic parvovirus H?1PV as a potential therapy against cervical and pancreatic carcinomas.'
31.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) 'Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration.'
32.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) "Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration." Transloncol. 6: 638-48.
33.) Li J, Bonifati S, Hristov G, Marttila T, Valmary?Degano S, Stanzel S, Schnolzer M, Mougin C, Aprahamian M, Grekova SP, Raykov Z, Rommelaere J, Marchini A. (2013) "Synergistic combination of valproic acid and oncolytic parvovirus H?1PV as a potential therapy against cervical and pancreatic carcinomas." EMBO Mol Med. 5: 1537-55.
34.) Abdelmoaty, S., Wigerblad, G., Bas, D.B., Codeluppi, S., Fernandez-Zafra, T., El-Awady, E.S., Moustafa, Y., Abdelhamid, A., Brodin, E. & Svensson, C.I. (2013) Spinal Actions of Lipoxin A4 and 17(R)-Resolvin D1 Attenuate Inflammation-Induced Mechanical Hypersensitivity and Spinal TNF Release PLoS One. 8
35.) Corti S, Nizzardo M, Simone C, Falcone M, Donadoni C, Salani S, Rizzo F, Nardini M, Riboldi G, Magri F, Zanetta C, Faravelli I,Bresolin N, Comi GP. (2012) "Direct reprogramming of human astrocytes into neural stem cells and neurons." Exp Cell Res. 318: 1528-41.
36.) Muller FL, Colla S, Aquilanti E, Manzo VE, Genovese G, Lee J, Eisenson D, Narurkar R, Deng P, Nezi L, Lee MA, Hu B, Hu J, Sahin E, Ong D, Fletcher-Sananikone E, Ho D, Kwong L, Brennan C, Wang YA, Chin L, DePinho RA. (2012) "Passenger deletions generate therapeutic vulnerabilities in cancer." Nature. 2488: 337-42
37.) Triscott J, Lee C, Hu K, Fotovati A, Berns R, Pambid M, Luk M, Kast RE, Kong E, Toyota E, Yip S, Toyota B, Dunn SE. (2012) "Disulfiram, a drug widely used to control alcoholism, suppresses the self-renewal of glioblastoma and over-rides resistance to temozolomide." Oncotarget. 3: 1112-23.
38.) Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D. (2011) "The role of shear stress in Blood-Brain Barrier endothelial physiology." BMC Neurosci. 12: 40.
39.) Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N. (2010) "Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow." Epilepsia. 51: 1408-17.
40.) Das A, Banik NL, Ray SK. (2010) "Flavonoids activated caspases for apoptosis in human glioblastoma T98G and U87MG cells but not in human normal astrocytes."Cancer. 116: 164-76.
41.) Furr SR, Moerdyk-Schauwecker M, Grdzelishvili VZ, Marriott I. (2010) "RIG-I mediates nonsegmented negative-sense RNA virus-induced inflammatory immune responses of primary human astrocytes." Glia. 58: 1620-9.
42.) Koob AO, Paulino AD, Masliah E. (2010) "GFAP reactivity, apolipoprotein E redistribution and cholesterol reduction in human astrocytes treated with alpha-synuclein."Neurosci Lett. 469: 11-4.
43.) Ruiz S, Brennand K, Panopoulos AD, Herrer as A, Gage FH, Izpisua-Belmonte JC. (2010) "High-efficient generation of induced pluripotent stem cells from human astrocytes." PLoS One. 5: e15526.
44.) Sanchez MC, Benitez A, Ortloff L, Green LM. (2009) "Alterations in glutamate uptake in NT2-derived neurons and astrocytes after exposure to gamma radiation." Radiat Res. 171: 41-52.
45.) Williams R, Dhillon NK, Hegde ST, Yao H, Peng F, Callen S, Chebloune Y, Davis RL, Buch SJ. (2009) "Proinflammatory cytokines and HIV-1 synergistically enhance CXCL10 expression in human astrocytes." Glia. 57: 734-43.
46.) Williams R, Yao H, Dhillon NK, Buch SJ. (2009) "HIV-1 Tat co-operates with IFN-gamma and TNF-alpha to increase CXCL10 in human astrocytes." PLoS One. 4: e5709.
47.) Hossain, M., Sathe, T., Fazio, V., Mazzone, P., Weksler, B., Janigro, D., Rapp, E. & Cucullo, L. (2009) Tobacco smoke: A critical etiological factor for vascular impairment at the blood?brain barrier Brain Res. 1287
48.) Cucullo L, Couraud P, Wekslet B, Romero I, Hossain M, Rapp E, Janigro D. (2008) "Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies." J Cereb Blood Flow Metab. 28: 312-28.
49.) Hoelzinger DB, Nakada M, Demuth T, Rosensteel T, Reavie LB, Berens ME. (2008) "Autotaxin: a secreted autocrine/paracrine factor that promotes glioma invasion." J Neurooncol. 86: 297-309.
50.) Qiu J, Ai L, Ramachandran C, Yao B, Gopalakrishnan S, Fields CR, Delmas AL, Dyer LM, Melnick SJ, Yachnis AT, Schwartz PH, Fine HA, Brown KD, Robertson KD. (2008) "Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas." Lab Invest. 88: 910-25.
51.) Cucullo L, Hossain M, Rapp E, Manders T, Marchi N, Janigro D. (2007) "Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic drugs." Epilepsia. 48: 505-16
52.) van den Pol AN, Robek MD, Ghosh PK, Ozduman K, Bandi P, Whim MD, Wollmann G. (2007) "Cytomegalovirus induces interferon-stimulated gene expression and is attenuated by interferon in the developing brain." J Virol. 81: 332-48.
53.) Konnikova L, Kotecki M, Kruger MM, Cochran BH. (2003) "Knockdown of STAT3 expression by RNAi induces apoptosis in astrocytoma cells." BMC Cancer. 3: 23.
54.)Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes,Eur J Neurosci. 2018 Jun;47(12):1444-1456. doi: 10.1111/ejn.13933. Epub 2018 May 30.
55.)Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways,Stem Cell Reports. 2019 Mar 5; 12(3): 488–501. Published online 2019 Feb 7. doi: 10.1016/j.stemcr.2019.01.003
56.)Interaction of tumor cells and astrocytes promotes breast cancer brain metastases through TGF-β2/ANGPTL4 axes,NPJ Precis Oncol. 2019; 3: 24.Published online 2019 Oct 3. doi: 10.1038/s41698-019-0094-1
57.)Hypoxia-enhanced blood-brain barrier chip recapitulates human barrier function and shuttling of drugs and antibodies,Nat Commun. 2019; 10: 2621.Published online 2019 Jun 13. doi: 10.1038/s41467-019-10588-0
58.)Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization,Nat Cell Biol. 2018 Aug; 20(8): 966–978.Published online 2018 Jul 23. doi: 10.1038/s41556-018-0138-8
59.)Cancer-cell-secreted exosomal miR-105 promotes tumour growth through the MYC-dependent metabolic reprogramming of stromal cells,Nat Cell Biol. 2018 May; 20(5): 597–609.Published online 2018 Apr 16. doi: 10.1038/s41556-018-0083-6
Approximately how many days does it take to go from initiating the culture to 90% confluence? How many cells can I expect from a 90% confluent T75 flask?
Answers:
Posted by Manoj Sharma on Wednesday, April 20, 2016
Hi,
it takes about 3 to 4 days to go from initiating the culture to 90% confluence and you can expect upto 5 to 6 millions cells.
reply for further question
thank you
Question:2
Hi, I have been using humans astrocytes from your company. I was wondering about the % of astrocytes that are GFAP positive to begin with. Also, do each vial represent cells from different donors ?
Answers:
Posted by Manoj Sharma on Wednesday, April 20, 2016
Hi Priya,
More than 95% of astrocytes are GFAP positive and each vial represent from single donor.
please reply for further questions
Thank You
Question:3
Which genes could represent good housekeeping genes for RT-qPCR analysis of these cells? Any suggestions?
Answers:
Posted by Karishma Shah on Tuesday, January 12, 2016
Hello Laurent,
We recommend β-actin, GAPDH, LDHA, NONO, and PPIH to serve as housekeeping genes for astrocyte qRT-PCR. We also recommend using multiple housekeeping genes for data normalization.
In addition, in a qRT-PCR assay, we recommend using Genomic DNA (gDNA) Control (GDC) to detect possible gDNA contamination, and Positive PCR Control (PPC) to test whether samples contain inhibitors or other factors that
may negatively affect gene expression results.
ScienCell offers GeneQuery™ Human Astrocyte Cell Biology qPCR Array Kit for astrocyte qRT-PCR analysis. For more information, please go to:
http://www.sciencellonline.com/products-services/gene-analysis/genequery-human-astrocyte-cell-biology-qpcr-array-kit.html
http://www.sciencellonline.com/PS/GK009.pdf
Thanks,
SCIENCELL TEAM
Question:4
Are this cell lines？
Answers:
Posted by Manoj Sharma on Thursday, January 7, 2016
Hello,
They are primary cells, not immortalized cell lines. thanks for your interest
SCIENCELL
Question:
What is the recommended maximum number of passages that the primary human astrocytes can be grown to without losing phenotype? Is there a recommended freezing protocol?
Answers:
Posted by Manoj Sharma on Thursday, January 7, 2016
Hi Srabasti,
Human Astrocytes can be subcultured for 4-5 passages. However, for primary cells, the markers such as GFAP may be lost during each passage, even though they are still astrocytes. Therefore, we recommend that customers use the cells at early passage without expanding or passaging too much. We do not recommend re-freezing the cells for future use.
thanks for your interest
SCIENCELL TEAM
上海中乔新舟生物科技有限公司
微信扫一扫咨询
