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SiHa人子宫颈鳞癌细胞(STR鉴定)[套餐促销]

英文名:SiHa
货号:ZQ0129
价格:¥1350.00
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SiHa人子宫颈鳞癌细胞(STR鉴定)[套餐促销]

¥1350.00
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SiHa人子宫颈鳞癌细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥1700 ¥2080.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

SiHa人子宫颈鳞癌细胞

货号

ZQ0129

产品介绍

SiHa细胞是一种人子宫颈鳞状细胞癌细胞系,几十年来在研究中被广泛使用。它们是从一位55岁患有鳞状细胞癌的日本女性患者的原始子宫活检碎片中分离出来的。这个细胞系因其独特的遗传特征,对研究宫颈癌和其他相关疾病的研究人员具有极大的兴趣。

SiHa细胞被发现表达p53+和pRB+基因,这些基因参与细胞周期调节、DNA修复和肿瘤抑制。这些基因使SiHa细胞成为研究癌症发展和进展的分子机制的理想模型。此外,SiHa细胞是适合的转染宿主,使它们成为基因表达研究的极好工具。

SiHa细胞具有超三倍体的核型,平均染色体数量在69到72之间。SiHa细胞是HPV-16阳性的,显示出每个细胞中有1到2份病毒基因组的整合。这些细胞具有致瘤性,在裸鼠中形成分化不良的表皮样癌(III级)。这使它们成为研究癌症进展和测试抗癌药物的极好模型。

SiHa细胞系表达多种同工酶,包括AK-1、ES-D、G6PD、GLO-I、Me-2、PGM1和PGM3。电子显微镜观察显示细胞质中有丰富的张力丝和细胞连接处的桥粒。SiHa细胞的生长特性是贴壁的,在10% FBS培养基中倍增时间为17小时,在5% FBS培养基中为21小时。上皮细胞黏附分子(EpCAM)在92%的SiHa细胞中表达,表明它们的上皮起源。它们显示出强烈的细胞角蛋白表达,但没有波形蛋白表达。
 

种属

性别/年龄

女/55岁

组织

子宫颈

疾病

二级,鳞状细胞癌

细胞类型

肿瘤细胞 

形态学

上皮细胞

生长方式

贴壁

倍增时间

大约21~60小时 

培养基和添加剂

MEM(含NEAA)(中乔新舟  货号:ZQ-300+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟   货号:CSP006

推荐完全培养基货号

ZM0129

生物安全等级

BSL-2

STR位点信息

Amelogenin: X

CSF1PO: 12
D13S317: 11
D16S539: 12
D5S818: 9
D7S820: 10
TH01: 6,9
TPOX: 8
vWA: 14,17
D3S1358: 16,17
D21S11: 29,31
D18S51: 15
Penta_E: 10,12
Penta_D: 9,12
D8S1179: 13,16
FGA: 21
D19S433: 14.2
D2S1338: 24

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; HTB-35

供应限制

仅供科研使用

货号

ZQ0129

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用



1、论文标题: The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer
DOI: 10.1186/s12885-022-09653-7
发表时间: 2022-06-09
期刊: BMC CANCER
影响因子: 4.43
货号: ZQ0129
产品名称: SiHa cells
原文链接: https://bmccancer.biomedcentral.com/articles/10.1186/s12885-022-09653-7


2、论文标题: Overexpression of SMC4 predicts a poor prognosis in cervical cancer and facilitates cancer cell malignancy phenotype by activating NF-κB pathway
DOI: 10.1007/s13577-021-00603-2
发表时间: 2021-09-03
期刊: Human Cell
影响因子: 4.174
货号: ZQ0129
产品名称: SiHa cells
原文链接: https://link.springer.com/article/10.1007/s13577-021-00603-2


3、论文标题: LncRNA SOX2OT affects cervical cancer cell growth, migration and invasion by regulating SOX2
DOI: 10.1080/15384101.2020.1750812
发表时间: 2020-04-14
期刊: CELL CYCLE
影响因子: 3.699
货号: ZQ0129
产品名称: SiHa cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/15384101.2020.1750812


4、论文标题: EGFR and ERK activation resists flavonoid quercetin‑induced anticancer activities in human cervical cancer cells <em>in vitro</em>
DOI: 10.3892/ol.2021.13015
发表时间: 2021-08-31
期刊: Oncology Letters
影响因子: 2.967
货号: ZQ0129
产品名称: SiHa cells
原文链接: https://www.spandidos-publications.com/10.3892/ol.2021.13015


5、论文标题: The degradation of Rap1GAP via E6AP-mediated ubiquitin-proteasome pathway is associated with HPV16/18-infection in cervical cancer cells
DOI: 10.1186/s13027-021-00409-9
发表时间: 2021-12-24
期刊: Infectious Agents and Cancer
影响因子: 2.965
货号: ZQ0129
产品名称: SiHa cells
原文链接: https://link.springer.com/article/10.1186/s13027-021-00409-9


6、论文标题: MiRNA-211 triggers an autophagy-dependent apoptosis in cervical cancer cells: regulation of Bcl-2
DOI: 10.1007/s00210-019-01720-4
发表时间: 2019-10-21
期刊: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
影响因子: 2.058
货号: ZQ0129
产品名称: SiHa cells

原文链接: https://link.springer.com/article/10.1007/s00210-019-01720-4


7、PubMed=20429727; DOI=10.3109/02841861003614343

Sorensen B.S., Toustrup K., Horsman M.R., Overgaard J., Alsner J.
Identifying pH independent hypoxia induced genes in human squamous cell carcinomas in vitro.
Acta Oncol. 49:895-905(2010)


8、PubMed=21439369; DOI=10.1016/j.mcp.2011.03.003
Schmitt M., Pawlita M.
The HPV transcriptome in HPV16 positive cell lines.
Mol. Cell. Probes 25:108-113(2011)


9、PubMed=22412903; DOI=10.1371/journal.pone.0032667
Vazquez-Mena O., Medina-Martinez I., Juarez-Torres E., Barron V., Espinosa A., Villegas-Sepulveda N., Gomez-Laguna L., Nieto-Martinez K., Orozco L., Roman-Bassaure E., Munoz Cortez S., Borges Ibanez M., Venegas-Vega C.A., Guardado-Estrada M., Rangel-Lopez A., Kofman S., Berumen J.
Amplified genes may be overexpressed, unchanged, or downregulated in cervical cancer cell lines.
PLoS ONE 7:E32667-E32667(2012)


10、PubMed=24134916; DOI=10.1186/1755-8166-6-44
McCormack A., Fan J.-L., Duesberg M., Bloomfield M., Fiala C., Duesberg P.H.
Individual karyotypes at the origins of cervical carcinomas.
Mol. Cytogenet. 6:44.1-44.23(2013)


11、PubMed=23881600; DOI=10.1002/jcp.24433
Ravi M., Sah S., Bhammar R.
Differences of SiHa (human cancer of cervix) and BMG-1 (brain glioma) cell lines as 2D and 3D cultures.
J. Cell. Physiol. 229:127-131(2014)


12、PubMed=24201445; DOI=10.1101/gr.164806.113
Akagi K., Li J.-F., Broutian T.R., Padilla-Nash H.M., Xiao W.-H., Jiang B., Rocco J.W., Teknos T.N., Kumar B., Wangsa D., He D., Ried T., Symer D.E., Gillison M.L.
Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability.
Genome Res. 24:185-199(2014)


13、PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)


14、PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)


15、PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)


16、PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)


17、PubMed=28261610; DOI=10.1155/2017/4180703
Kontostathi G., Zoidakis J., Makridakis M., Lygirou V., Mermelekas G., Papadopoulos T., Vougas K., Vlamis-Gardikas A., Drakakis P., Loutradis D., Vlahou A., Anagnou N.P., Pappa K.I.
Cervical cancer cell line secretome highlights the roles of transforming growth factor-beta-induced protein ig-h3, peroxiredoxin-2, and NRF2 on cervical carcinogenesis.
Biomed Res. Int. 2017:4180703.1-4180703.15(2017)


18、PubMed=29156801; DOI=10.18632/oncotarget.21174
Kalu N.N., Mazumdar T., Peng S.-H., Shen L., Sambandam V., Rao X.-Y., Xi Y.-X., Li L.-R., Qi Y., Gleber-Netto F.O., Patel A., Wang J., Frederick M.J., Myers J.N., Pickering C.R., Johnson F.M.
Genomic characterization of human papillomavirus-positive and -negative human squamous cell cancer cell lines.
Oncotarget 8:86369-86383(2017)


19、PubMed=30760827; DOI=10.1038/s41598-018-38315-7
Rosa M.N., Evangelista A.F., Leal L.F., de Oliveira C.M., Silva V.A.O., Munari C.C., Munari F.F., de Macedo Matsushita G., dos Reis R., Andrade C.E., de Padua Souza C., Reis R.M.
Establishment, molecular and biological characterization of HCB-514: a novel human cervical cancer cell line.
Sci. Rep. 9:1913-1913(2019)


20、PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)


21、PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)


22、PubMed=35198691; DOI=10.1016/j.dib.2022.107919
Huang Z.-L., Kong W.-J., Wong B.J.-H., Gao H.-H., Guo T.-N., Liu X.-M., Du X.-X., Wong L.-S., Goh W.W.-B.
Proteomic datasets of HeLa and SiHa cell lines acquired by DDA-PASEF and diaPASEF.
Data Brief 41:107919.1-107919.7(2022)


23、PubMed=35421932; DOI=10.1186/s12885-022-09528-x
He Y., Han S.-B., Liu Y., Zhang J.-J., Wu Y.-M.
Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer.
BMC Cancer 22:411.1-411.12(2022)


24、PubMed=35692560; DOI=10.1080/23723556.2022.2078628
de Lourdes Zuniga-Martinez M., Lopez-Mendoza C.M., Tenorio-Salazar J., Garcia-Carranca A.M., Cerbon-Cervantes M.A., Alcantara-Quintana L.E.
Establishment, authenticity, and characterization of cervical cancer cell lines.
Mol. Cell. Oncol. 9:2078628.1-2078628.10(2022)


25、PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)



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