产品名称 |
HK-2人肾上皮细胞 |
货号 |
ZQ0313 |
产品介绍 |
HK-2 细胞系是一种特征明确的人类近端小管上皮细胞系,来源于正常成人肾脏组织。通过用人乳头瘤病毒16 (HPV-16) E6/E7基因转导使细胞永生化。根据Southern和FISH分析,该细胞系似乎来自单个细胞。这些细胞表现出典型的上皮形态,并保留了近端小管细胞的许多生化和功能特性,使其成为研究肾脏生理学和病理生理学的宝贵模型。HK-2 细胞以其进行主动运输和表现出刷状缘酶活性的能力而闻名,这对其在肾脏重吸收过程中的作用至关重要。
特别注意: 请使用0.05%胰酶消化细胞。同时由于我司培养液为低血清配方,故无法终止胰酶消化,消化时完培不能当做终止液,需要用0.1%大豆胰蛋白酶抑制剂(号:CSP002)终止消化。 |
种属 |
人 |
性别/年龄 |
男/成年人 |
组织 |
肾、皮质/近端小管 |
疾病 |
乳头(状)瘤 |
细胞类型 |
human papillomavirus 16 (HPV-16) transformed |
形态学 |
上皮的 |
生长方式 |
贴壁 |
倍增时间 |
大约48~72小时 |
培养基和添加剂 |
500ml基础培养基;5 ml 胎牛血清; 5ml细胞生长因子;5ml青霉素/链霉素溶液 (备注:每种组分单独包装,使用前需要按比例分装,详细操作详见说明书,现用现配,效果更佳。) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-2 |
STR位点信息 |
Amelogenin: X,Y CSF1PO: 13 D13S317: 9 D16S539: 11,12 D5S818: 12 D7S820: 10,11 TH01: 9 TPOX: 8,9 vWA: 17,18 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2190 BCRC; 60097 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0313 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题: Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors
DOI: 10.1136/jitc-2022-004691
发表时间: 2022-05-01
期刊: Journal for ImmunoTherapy of Cancer
影响因子: 13.751
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://jitc.bmj.com/content/10/5/e004691.abstract
论文标题: DsbA-L interacts with VDAC1 in mitochondrion-mediated tubular cell apoptosis and contributes to the progression of acute kidney disease
DOI: 10.1016/j.ebiom.2022.103859
发表时间: 2022-02-04
期刊: EBioMedicine
影响因子: 8.143
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S2352396422000433
论文标题: Kidney extracellular matrix hydrogel enhances therapeutic potential of adipose-derived mesenchymal stem cells for renal ischemia reperfusion injury
DOI: 10.1016/j.actbio.2020.07.056
发表时间: 2020-08-06
期刊: Acta Biomaterialia
影响因子: 7.242
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S1742706120304530
论文标题: Asiatic acid from Cyclocarya paliurus regulates the autophagy–lysosome system via directly inhibiting TGF-β type I receptor and ameliorates diabetic nephropathy fibrosis
DOI: 10.1039/D1FO02445K
发表时间: 2022-04-15
期刊: Food & Function
影响因子: 5.396
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo02445k
论文标题: Knockdown of periostin attenuates 5/6 nephrectomy‐induced intrarenal renin–angiotensin system activation, fibrosis, and inflammation in rats
DOI: 10.1002/jcp.28849
发表时间: 2019-05-24
期刊: JOURNAL OF CELLULAR PHYSIOLOGY
影响因子: 4.522
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28849
论文标题: Nobiletin downregulates the SKP2-p21/p27-CDK2 axis to inhibit tumor progression and shows synergistic effects with palbociclib on renal cell carcinoma
DOI: 10.20892/j.issn.2095-3941.2020.0186
发表时间: 2021-02-15
期刊: Cancer Biology & Medicine
影响因子: 4.248
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877181/
论文标题: Circadian clock gene BMAL1 reduces urinary calcium oxalate stones formation by regulating NRF2/HO-1 pathway
DOI: 10.1016/j.lfs.2020.118853
发表时间: 2020-12-02
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0313
产品名称: HK2 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520316064
论文标题: Realgar induced nephrotoxicity via ferroptosis in mice
DOI: 10.1002/jat.4362
发表时间: 2022-07-08
期刊: JOURNAL OF APPLIED TOXICOLOGY
影响因子: 3.628
货号: ZQ0313
产品名称: HK2 cells
原文链接: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jat.4362
论文标题: Pioglitazone attenuates kidney fibrosis via miR-21-5p modulation
DOI: 10.1016/j.lfs.2019.116609
发表时间: 2019-06-27
期刊: LIFE SCIENCES
影响因子: 3.448
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320519305351
论文标题: Inhibition of microRNA-182-5p contributes to attenuation of lupus nephritis via Foxo1 signaling
DOI: 10.1016/j.yexcr.2018.09.026
发表时间: 2018-10-09
期刊: EXPERIMENTAL CELL RESEARCH
影响因子: 3.309
货号: ZQ0313
产品名称: HK-2 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0014482718310383
论文标题: Epigenetic inactivation of hydroxymethylglutaryl CoA synthase reduces ketogenesis and facilitates tumor cell motility in clear cell renal carcinoma
DOI: 10.1016/j.prp.2021.153622
发表时间: 2021-09-20
期刊: PATHOLOGY RESEARCH AND PRACTICE
影响因子: 3.25
货号: ZQ0313
产品名称: Human renal epithelial cell line HK-2
原文链接: https://www.sciencedirect.com/science/article/pii/S0344033821002831
论文标题: Spleen tyrosine kinase promotes NLR family pyrin domain containing 3 inflammasome‑mediated IL‑1β secretion via c‑Jun N‑terminal kinase activation and cell apoptosis during diabetic nephropathy
DOI: 10.3892/mmr.2018.9164
发表时间: 2018-06-14
期刊: Molecular Medicine Reports
影响因子: 1.922
货号: ZQ0313
产品名称: HK2 cells
原文链接: https://www.spandidos-publications.com/mmr/18/2/1995
论文标题: Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells
DOI: 10.1016/j.acthis.2019.06.005
发表时间: 2019-06-22
期刊: ACTA HISTOCHEMICA
影响因子: 1.719
货号: ZQ0313
产品名称: HK2 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0065128119301321
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HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney.
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Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
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An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
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Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
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Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)
货号 | 产品名称 | 规格 | 价格 | 指令 |
2101 | 角质细胞培养基 | 500ML | ¥2232.00 | 放入购物车 》 |
ZQ0194 | SV40 MES 13小鼠肾小球系膜细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1200.00 | 放入购物车 》 |
ZQ0195 | Y1小鼠肾上腺皮质瘤细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1000.00 | 放入购物车 》 |
ZQ0410 | SW-13人肾上腺皮质小细胞癌细胞 (STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
ZQ500 | 国产优级胎牛血清 | 500ml | ¥1800.00 | 放入购物车 》 |
6 | 绿/红色荧光蛋白标记 | ¥询价 | 放入购物车 》 |