产品名称 |
SK-NEP-1人肾母细胞瘤细胞 |
货号 |
ZQ0337 |
产品介绍 |
SK-NEP-1细胞系是一种人肾母细胞瘤细胞系,源自一位25岁白人女性的肾组织,取自肾母细胞瘤和胸膜渗出液。该细胞系在培养条件下呈上皮样形态,圆形,具有少量微绒毛、连接复合体,以及发育良好的高尔基体和光滑内质网,细胞还含有脂滴,无病毒颗粒。
注意事项: |
种属 |
人 |
性别/年龄 |
女/25岁 |
组织 |
肾;来源于转移部位: 胸腔积液 |
疾病 |
尤因肉瘤 |
细胞类型 |
肿瘤细胞 |
形态学 |
圆形的 |
生长方式 |
悬浮 |
倍增时间 |
大约72小时 |
培养基和添加剂 |
McCOY's 5A(中乔新舟 货号:ZQ-1000)+15%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,10 TH01: 8,9.3 TPOX: 8,11 vWA: 15,19 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; HTB-48 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0337 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
DOI=10.1007/978-1-4757-1647-4_5
Fogh J., Trempe G.L.
New human tumor cell lines.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.115-159; Springer; New York (1975)
PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)
PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)
PubMed=571047
Fogh J.
Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.
Natl. Cancer Inst. Monogr. 49:5-9(1978)
PubMed=6244232
Williams R.D.
Human urologic cancer cell lines.
Invest. Urol. 17:359-363(1980)
PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)
PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)
PubMed=17154184; DOI=10.1002/pbc.21099
Smith M.A., Morton C.L., Phelps D.A., Girtman K., Neale G., Houghton P.J.
SK-NEP-1 and Rh1 are Ewing family tumor lines.
Pediatr. Blood Cancer 50:703-706(2008)
PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)
PubMed=25010205; DOI=10.1371/journal.pgen.1004475
Brohl A.S., Solomon D.A., Chang W., Wang J.-J., Song Y., Sindiri S., Patidar R., Hurd L., Chen L., Shern J.F., Liao H.-L., Wen X.-Y., Gerard J., Kim J.-S., Lopez Guerrero J.A., Machado I., Wai D.H., Picci P., Triche T.J., Horvai A.E., Miettinen M.M., Wei J.S., Catchpoole D., Llombart-Bosch A., Waldman T., Khan J.
The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:E1004475-E1004475(2014)
PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=28489074; DOI=10.1038/ncomms15165
Sinha R., Winer A.G., Chevinsky M., Jakubowski C., Chen Y.-B., Dong Y.-Y., Tickoo S.K., Reuter V.E., Russo P., Coleman J.A., Sander C., Hsieh J.J.-D., Hakimi A.A.
Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection.
Nat. Commun. 8:15165.1-15165.10(2017)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
PubMed=30924592; DOI=10.1002/pbc.27741
Pritchard-Jones K., Perotti D.
WARNING: G-401 and SK-NEP-1 cell lines are not Wilms tumor cell lines.
Pediatr. Blood Cancer 66:e27741.1-e27741.2(2019)
PubMed=31006967; DOI=10.1002/pbc.27769
Staege M.S.
Are EWSR1-FLI-positive cell lines from patients with other diagnoses than Ewing sarcoma really Ewing sarcoma cell lines?
Pediatr. Blood Cancer 66:e27769.1-e27769.2(2019)
PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0418 | HMy2.CIR人B淋巴母细胞 (STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0429 | MOLT-4人急性淋巴母细胞白血病细胞 | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
5 | 荧光素酶标记 | ¥询价 | 放入购物车 》 | |
6 | 绿/红色荧光蛋白标记 | ¥询价 | 放入购物车 》 | |
ZQ-1000 | McCoy's 5A 基础培养基 | 500ml | ¥96.00 | 放入购物车 》 |
ZQ-1002 | McCoy's 5A 完全培养基 | 500ml | ¥750.00 | 放入购物车 》 |