产品名称
HEC-1-B人子宫内膜腺癌细胞
货号
ZQ0364
产品介绍
HEC-1-B细胞系是一种人子宫内膜腺癌细胞系,这是 HEC-1-A 的一个亚株。与 HEC-1-A 不同,该亚株在培养的第 135 天和第 190 天之间表现出稳定的生长期,恢复后看起来比亲本系更平坦且具有更多的路面图案。染色体的主要互补体是亲本观察到的两倍线。该细胞已广泛应用于与子宫内膜癌、激素反应和癌症药理学研究相关的生物医学研究中。已知该细胞表达雌激素和孕激素受体,使其成为研究子宫内膜癌进展和治疗中激素相关动力学的有价值的模型。这些细胞已被用于研究癌细胞增殖、分化以及对激素和化疗治疗的反应的分子机制。
种属
人
性别/年龄
女/71岁
组织
子宫;子宫内膜
疾病
腺癌
细胞类型
肿瘤细胞
形态学
上皮
生长方式
贴壁
倍增时间
大约72~96小时
培养基和添加剂
MEM(含NEAA)(中乔新舟 货号:ZQ-300)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006)
推荐完全培养基货号
生物安全等级
BSL-1
STR位点信息
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,16
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
TH01: 6,7
TPOX: 8,11
vWA: 18
培养条件
95%空气,5%二氧化碳;37℃
抗原表达/受体表达
***
基因表达
***
保藏机构
ATCC; HTB-113 JCRB; JCRB1193 JCRB; NIHS0480
供应限制
仅供科研使用
在形态学上,HEC-1-B细胞呈典型的上皮样形态,呈单层生长。其特点是体外增殖能力强。遗传研究揭示了一些染色体的改变,这些改变被认为是导致这些细胞癌变表型的原因。使用HEC-1-B细胞系的研究有助于更深入地了解子宫内膜癌的发生,并为测试潜在的治疗药物提供了一个强大的系统。该细胞系也通常用于专注于癌细胞入侵和转移的研究,为支持这些过程的细胞行为提供了见解。
货号 |
ZQ0364 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题: Dachsous cadherin related 1 (DCHS1) is a novel biomarker for immune infiltration and epithelial-mesenchymal transition in endometrial cancer via pan-cancer analysis
DOI:10.1186/s13048-024-01478-1.
发表时间: 2024-8-9
期刊: Journal of Ovarian Research
影响因子: 3.8
货号: ZQ0364
产品名称:HEC-1B cells
原文链接:https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-024-01478-1
论文标题: Synaptotagmin-like protein 1 is a potential diagnostic and prognostic biomarker in endometrial cancer based on bioinformatics and experiments
DOI:10.1186/s13048-023-01097-2.
发表时间: 2023-1-19
期刊: Journal of Ovarian Research
影响因子: 3.8
货号: ZQ0364
产品名称: HEC-1B cells
原文链接:https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-023-01097-2
论文标题: UBE2C is upregulated by estrogen and promotes epithelial-mesenchymal transition via p53 in endometrial cancer
DOI: 10.1158/1541-7786.MCR-19-0561
发表时间: 2019-01-01
期刊: MOLECULAR CANCER RESEARCH
影响因子: 4.484
货号: ZQ0364
产品名称: HEC-1B cells
原文链接: https://mcr.aacrjournals.org/content/18/2/204.abstract
PubMed=4678779
Kuramoto H.
Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line.
Acta Obstet. Gynaecol. Jpn. 19:47-58(1972)
PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)
PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)
PubMed=6163305
Sekiya S., Takamizawa H.
Effect of anticancer drugs on in vitro survival of cell lines derived from various gynecologic tumors.
Nihon Sanka Fujinka Gakkai Zasshi 33:373-376(1981)
PubMed=6174649
Sekiya S., Kawata M., Inaba N., Takamizawa H.
Studies on marker substances in cell lines derived from various human gynecologic tumors.
Nihon Sanka Fujinka Gakkai Zasshi 34:37-42(1982)
PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)
PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)
PubMed=12227503; DOI=10.1111/j.1749-0774.2002.tb00103.x
Kuramoto H., Hamano M., Imai M.
HEC-1 cells.
Hum. Cell 15:81-95(2002)
DOI=10.1007/978-4-431-53981-0_1
Kuramoto H., Hamano M., Imai M., Fujisawa T., Kamata Y., Arai T., Kawaguchi M.
HEC-1 cells: establishment of an in vitro experimental system in endometrial carcinoma.
(In) Cell and molecular biology of endometrial carcinoma; Kuramoto H., Nishida M. (eds.); pp.3-34; Springer; Tokyo (2003)
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)
PubMed=20218740; DOI=10.1177/153303461000900207
Wang Y.-M., Yang D., Cogdell D., Hu L.-M., Xue F.-X., Broaddus R., Zhang W.
Genomic characterization of gene copy-number aberrations in endometrial carcinoma cell lines derived from endometrioid-type endometrial adenocarcinoma.
Technol. Cancer Res. Treat. 9:179-189(2010)
PubMed=20944090; DOI=10.1126/scitranslmed.3001538
Dedes K.J., Wetterskog D., Mendes-Pereira A.M., Natrajan R., Lambros M.B., Geyer F.C., Vatcheva R., Savage K., Mackay A., Lord C.J., Ashworth A., Reis-Filho J.S.
PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors.
Sci. Transl. Med. 2:53ra75.1-53ra75.8(2010)
PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)
PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)
PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)
PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0342 | HEC-1-A人子宫内膜腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1400.00 | 放入购物车 》 |
ZQ0362 | RL95-2人子宫内膜癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1350.00 | 放入购物车 》 |
ZQ0471 | AN3CA人子宫内膜癌细胞 (STR鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
6 | 绿/红色荧光蛋白标记 | ¥询价 | 放入购物车 》 | |
ZQ-300 | MEM(含有NEAA)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-301 | EMEM(含有NEAA)完全培养基 | 500ml | ¥350.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |
JSFW-134 | 人源细胞STR鉴定服务 | ¥900.00 | 放入购物车 》 | |
ZQ0472 | ISK人子宫内膜癌细胞[套餐促销] | 5 x 10^5 cells/vial | ¥1400.00 | 放入购物车 》 |