您是第 10436640 位 欢迎访问 中乔新舟官网 ! 全国免费电话:400-038-9959 我的购物车(0) 注 册 / 登 录
扫码关注公众号
您当前的位置:首页 > 产品中心

HBL-100人整合SV40基因的乳腺上皮细胞(STR鉴定)

英文名:HBL-100
货号:ZQ0370
价格:¥1000.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
推荐组合

HBL-100人整合SV40基因的乳腺上皮细胞(STR鉴定)

¥1000.00
+

HBL-100人整合SV40基因的乳腺上皮细胞专用培养基

¥350.00 ¥450.00

配套完培,组合使用,省时省力!

=

细胞套餐惊爆价

¥1350 ¥1450.00
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

HBL-100人整合SV40基因的乳腺上皮细胞

货号

ZQ0370

产品介绍

HBL-100是一种人类乳腺上皮细胞系,最初来源于哺乳期母亲的母乳。母乳是在分娩后三天收集的,尽管供体没有乳房病变的证据,也没有乳腺癌家族史,但细胞在第7代时表现出异常的核型。该细胞系以其合成少量乳糖的能力和通过增加酪蛋白的产生对催乳素或雌激素的刺激作出反应而闻名。显微镜分析,如电子显微镜,证实了这些细胞中存在微绒毛、张力原纤维和桥粒,突出了它们典型的上皮特征。
然而,HBL-100细胞系在其鉴定和表征方面遇到了重大的并发症。它被发现含有一条Y染色体,这表明一种错误的识别,因为细胞系最初被认为是女性起源。进一步的复杂性来自细胞系中SV40基因组序列的存在,这与早期认为它是自发不朽的观点相矛盾。这些发现引发了关于HBL-100的起源和基因组成的争论,使其成为一个有问题的细胞系,没有对其特征和起源进行彻底的验证。

注意事项:
HBL-100细胞低密度时细胞状态要特别注意温度变化和和细胞震动,否则细胞易脱落,在细胞密度80%时细胞即可传代。建议使用Corning CellBIND(3289)培养瓶。

种属

性别/年龄

组织

整合SV40基因的乳腺上皮细胞

细胞类型

转化细胞系 

形态学

上皮

生长方式

贴壁

倍增时间

大约28~40小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200+20%胎牛血清(中乔新舟 货号:ZQ0500+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0370

生物安全等级

BSL-1

STR位点信息

Amelogenin: X
D5S818:11,12
D13S317: 12
D7S820: 8,12
D16S539: 9,12
vWA: 16
TH01: 6,8
TPOX: 8
CSF1PO: 10
D19S433: 15
D21S11: 28,30
D18S51: 16 

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; HTB-124 RCB; RCB0460

供应限制

仅供科研使用

货号

ZQ0370

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


CLPUB00368
Polanowski F.P., Gaffney E.V., Burke R.E.
HBL-100, a cell line established from human breast milk.
In Vitro 12:328-328(1976)


PubMed=6891286; DOI=10.1007/BF00204786
Gaffney E.V.
A cell line (HBL-100) established from human breast milk.
Cell Tissue Res. 227:563-568(1982)


PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)


PubMed=2995096; DOI=10.1016/0014-4827(85)90238-1
Caron de Fromentel C., Nardeux P.-C., Soussi T., Lavialle C., Estrade S., Carloni G., Chandrasekaran K., Cassingena R.
Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information.
Exp. Cell Res. 160:83-94(1985)


PubMed=3109929; DOI=10.1016/0014-4827(87)90318-1
Dealtry G.B., Naylor M.S., Fiers W.C., Balkwill F.R.
The effect of recombinant human tumour necrosis factor on growth and macromolecular synthesis of human epithelial cells.
Exp. Cell Res. 170:428-438(1987)


PubMed=2840847
Marlhens F., Saint-Ruf C., Nardeux P.-C., Lavialle C., Brouty-Boye D., Dutrillaux B., Cassingena R.
Karyotype evolution of the human HBL-100 cell line and mapping of the integration site of SV40 DNA.
Ann. Genet. 31:81-86(1988)


PubMed=2547730; DOI=10.1002/ijc.2910440230
Saint-Ruf C., Nardeux P.-C., Cebrian J., Lacasa M., Lavialle C., Cassingena R.
Molecular cloning and characterization of endogenous SV40 DNA from human HBL-100 cells.
Int. J. Cancer 44:367-372(1989)


DOI=10.1016/B978-0-12-333530-2.50009-5
Leibovitz A.
Cell lines from human breast.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York (1994)


PubMed=9671407; DOI=10.1038/sj.onc.1201814
Sweeney K.J., Swarbrick A., Sutherland R.L., Musgrove E.A.
Lack of relationship between CDK activity and G1 cyclin expression in breast cancer cells.
Oncogene 16:2865-2878(1998)


PubMed=10969801
Forozan F., Mahlamaki E.H., Monni O., Chen Y.-D., Veldman R., Jiang Y., Gooden G.C., Ethier S.P., Kallioniemi A., Kallioniemi O.-P.
Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.
Cancer Res. 60:4519-4525(2000)


PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)


PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)


PubMed=16643607; DOI=10.1111/j.1749-0774.2005.00007.x
Yoshino K., Iimura E., Saijo K., Iwase S., Fukami K., Ohno T., Obata Y., Nakamura Y.
Essential role for gene profiling analysis in the authentication of human cell lines.
Hum. Cell 19:43-48(2006)


PubMed=17157791; DOI=10.1016/j.ccr.2006.10.008
Neve R.M., Chin K., Fridlyand J., Yeh J., Baehner F.L., Fevr T., Clark L., Bayani N., Coppe J.-P., Tong F., Speed T., Spellman P.T., DeVries S., Lapuk A., Wang N.J., Kuo W.-L., Stilwell J.L., Pinkel D., Albertson D.G., Waldman F.M., McCormick F., Dickson R.B., Johnson M.D., Lippman M.E., Ethier S.P., Gazdar A.F., Gray J.W.
A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.
Cancer Cell 10:515-527(2006)



CLPUB00423

Morrison B.J.
Breast cancer stem cells: tumourspheres and implications for therapy.
Thesis PhD (2010), Griffith University, Australia



PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)


PubMed=24094812; DOI=10.1016/j.ccr.2013.08.020
Timmerman L.A., Holton T., Yuneva M., Louie R.J., Padro M., Daemen A., Hu M., Chan D.A., Ethier S.P., van 't Veer L.J., Polyak K., McCormick F., Gray J.W.
Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.
Cancer Cell 24:450-465(2013)


PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)


PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)


PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)


PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)


PubMed=28889351; DOI=10.1007/s10549-017-4496-x
Saunus J.M., Smart C.E., Kutasovic J.R., Johnston R.L., Kalita-de Croft P., Miranda M., Rozali E.N., Vargas A.C., Reid L.E., Lorsy E., Cocciardi S., Seidens T., McCart Reed A.E., Dalley A.J., Wockner L.F., Johnson J., Sarkar D., Askarian-Amiri M.E., Simpson P.T., Khanna K.K., Chenevix-Trench G., Al-Ejeh F., Lakhani S.R.
Multidimensional phenotyping of breast cancer cell lines to guide preclinical research.
Breast Cancer Res. Treat. 167:289-301(2018)


公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
           上海中乔新舟生物科技有限公司(官网:www.zqx...
联系我们 / Contact
电 话:021-56760357;021-56760351
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved. 网站地图

沪公网安备 31011002001038号

技术支持:攸攸网络 沪ICP备14008091号