胰岛素铁硒传递蛋白描述:
ITS is a mixture of insulin, transferrin, and selenium. It is a general cell supplement designed for use in classical media such as DMEM, RPMI-1640 and nutrient media such as Ham’s F-12, DMEM/F-12. ITS enhances cell proliferation and decreases the serum requirement of many cell types [1, 2, 3]. When ITS is used with a low percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
ITS is prepared in Hank’s Balanced Salt Solution (HBSS) without phenol red.
Formula
Insulin 1.0 mg/ml
Apo-Transferrin 1.0 mg/ml
Sodium Selenite 3.4 µM
产品使用说明:仅供科研研究使用
货号 |
0803 |
产地 |
美国 |
缩写 |
ITS |
规格 |
10ml |
用途 |
科研 |
包装 |
管装 |
保存 |
-20°C |
运输 |
干冰 |
参考文献 |
1. Guilbert, L. J. and Iscove, N. N. (1976)
Partial replacement of serum be selenite, transferrin, albumin, and lecithin
onhaemopoietic cell culture. Nature, 263: 594-595.
2. Kelley, D. S.,
Becker, J. E., and Porter, V. R. (1978) Effects of insulin, dexamethasone,
and glucagon on the amino acid transport ability of four rat hepatoma cell
lines and rat hepatocytes in culture. Cancer Res., 38: 4591- 4601. s, NY, p.
103.
3. McKeehan, W.
L., Hamilton, W. G., and Ham, R. G. (1976) Selenium is an essential trace nutrient
for the growth of WI-38 diploid human fibroblasts. Proc. Natl. Acad. Sci.,
USA 73: 2023-2027.
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