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人吞噬作用定量PCR芯片试剂盒
英文名:GeneQuery™ Human Phagocytosis qPCR Array Kit
货号:GK038
价格:¥4320.00
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人吞噬作用定量PCR芯片试剂盒描述:



       ScienCell's GeneQuery™ Human Phagocytosis qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in phagocytosis during apoptosis or the immune response. Brief examples of how included genes may be grouped according to function are shown below:


       Apoptotic phagocytosis: MSR1, RAB22A, ITGB3, MARCO, PTGES2

       Immune responsive phagocytosis: FGR, SYK, LYN, ARHGAP1, MARCKS

       Necrosis detection: TLR2, TLR5, TLR6, IL1R1, SARM1

       Protein digestion: CTSB, CTSA, VAMP4, ANPEP, FTH1

       Lipid digestion and storage: CLN5, LPL, LRP12, NPC2


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.


       GeneQuery™ Human Phagocytosis qPCR Array Plate Layout* (8 controls in Bold and Italic)



 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTB

CALM1

CTSB

DNM1

GAPVD1

ITGB3

MARCO

NOX1

RAB1A

RABGEF1

TLR2

β-actin

B

ACTG1

CALR

CTSA

EDEM2

HMOX2

KLC1

MRC1

NOXA1

RAB22A

RAC1

TLR3

GAPDH

C

ANPEP

CAMK1

CTSB

FCGR1A

IL12A

LAMP1

MRC2

NPC2

RAB33A

RAP1A

TLR4

LDHA

D

ANXA1

CCL2

CTSH

FCGR2B

IL12B

LAMP2

MSR1

PIK3R1

RAB4A

RILP

TLR5

NONO

E

ANXA2

CD14

CTSS

FCGR3A

IL1B

LPL

MYL2

PPARG

RAB5A

SARM1

TLR6

PPIH

F

ANXA4

CD36

CXCL2

FCGR3B

IL1R1

LRP12

NAGK

PRKCA

RAB5B

SYK

TNF

GDC

G

ARHGAP1

CLN5

CXCL8

FGR

IL6

LYN

NFKB1

PTGES2

RAB7A

TLN1

VAMP4

PPC

H

BLVRB

CR1

DCTN1

FTH1

ITGAV

MARCKS

NFKB2

RAB14

RAB9A

TLN2

VCL

NTC

 

*gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK038

产地

美国

缩写

GQH-PHG

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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