人多能干细胞定量PCR芯片试剂盒描述：

       ScienCell's GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on database and literature research.      

       GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

       GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

产品使用说明:仅供科研研究使用

Question:

       1. What things should I add in Positive PCR Control (PPC) ? Customers’ samples or not ? 2. "replicates are strongly recommended (minimum of 3)." Should I buy 3 qPCR Array Kits at least?

Answers:

       Posted by Karishma Shah on Thursday, September 24, 2015

       Hi Sophia, thanks for asking!

       1. PPC control contains a DNA template and a primer set that can amplify it. To verify that qPCR program is working, the 2 components need to add are H2O and qPCR master mix of customer's choice. To be able to detect if there is possible PCR inhibitors in the cDNA samples, cDNA sample, H2O and qPCR master mix should all be added to the PPC well.

       2. Depending on the purpose of the experiments, and the number of samples to compare, the number of kits needed will be based on customer's experiment design.

       Thanks!