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人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:
Cell cycle regulation: CCNT1, CCNT2, CDK9, CDK14, CDKN2C
Signaling mediation: IGF1R, BMP2K, PROM1
Differentiation regulation: CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
Mutated transcription factors identified
in acute myeloid leukemia: AML1, CEBPA, PLZF, ZBTB16, SPI1
in precursor B lymphoblastic leukemia: TCF3, PBX1
in precursor T lymphoblastic leukemia: TLX1, TLX3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AFF1 |
CCND2 |
CDKN1A |
DUSP6 |
HDAC1 |
HPGD |
KLF3 |
MEIS1 |
PBX1 |
RUNX2 |
TCF3 |
β-actin |
|
B |
ATM |
CCNT1 |
CDKN2C |
ETV6 |
HDAC2 |
IGF1 |
KMT2A |
MLLT1 |
PER2 |
SIN3A |
TLX1 |
GAPDH |
|
C |
BCL2A1 |
CCNT2 |
CEBPA |
ETV7 |
HHEX |
IGF1R |
LDB1 |
MLLT3 |
PROM1 |
SIX1 |
TLX3 |
LDHA |
|
D |
BCL2L1 |
CCR7 |
CSF1R |
EYA1 |
HIST2H3C |
IL3 |
LMO2 |
MYC |
PTCRA |
SIX4 |
TRAF1 |
NONO |
|
E |
BCL6 |
CD14 |
CSF2 |
FCGR1A |
HMGA2 |
ITGB7 |
LYL1 |
MYCN |
RARA |
SMAD1 |
UTY |
PPIH |
|
F |
BIRC3 |
CD86 |
DDIT3 |
FUT8 |
HOXA10 |
JMJD1C |
MAF |
NCOR1 |
REL |
SP1 |
WHSC1 |
GDC |
|
G |
BMP2K |
CDK14 |
DEFA3 |
GRIA3 |
HOXA11 |
JUP |
MAX |
NFKB1 |
RUNX1 |
SPI1 |
WNT16 |
PPC |
|
H |
CCNA1 |
CDK9 |
DOT1L |
GZMB |
HOXA9 |
KDM6A |
MEF2C |
PAX5 |
RUNX1T1 |
SUPT3H |
ZBTB16 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK030 |
|
产地 |
美国 |
|
缩写 |
GQH-TMH |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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