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人黑色素瘤定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ human melanoma qPCR array kit (GQH-MEL) is designed to facilitate gene expression profiling of human melanoma related genes. Melanoma, also known as malignant melanoma, is a type of malignant cancer that results from uncontrolled growth of melanocytes, which are melanin-producing, pigmented cells. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery Human Melanoma qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ABCC2 |
BRAF |
CTGF |
EGFR |
GNAQ |
ITGA7 |
MITF |
OPN |
PPP2R2C |
SHOX2 |
TM4SF13 |
β-actin |
|
B |
AKT1 |
BRCA2 |
CTNNB1 |
FABP7 |
HOXB7 |
ITGB3 |
MLANA |
MAPK14 |
PTPRF |
SNAI2 |
TMEM229B |
GAPDH |
|
C |
AKT2 |
BRN2 |
CXCR4 |
FGF13 |
HRAS |
KIT |
MX1 |
PAX3 |
Rab5b |
SNAI1 |
TNC |
LDHA |
|
D |
AKT3 |
CDH1 |
CYR61 |
FGFR2 |
IGF1 |
KRAS |
Necdin |
PIK3CA |
RB1 |
SOS1 |
TP53 |
NONO |
|
E |
ASXL1 |
CDKN2A |
DNAJA1 |
FGFR3 |
IGFBP3 |
LIF |
NOTCH2 |
PIK3CB |
RNF128 |
SOX10 |
TPD52 |
PPIH |
|
F |
BCL2 |
CHP2 |
DTX3L |
FOXD1 |
ISG15 |
MAPK3 |
NPM2 |
PIK3CD |
RPS6KA1 |
STK11 |
TYRP1 |
GDC |
|
G |
BCL2L1 |
COL11A1 |
EDNRB |
FOXD3 |
ITGA4 |
MC1R |
NRAS |
PLA1A |
RUNX3 |
TIMP3 |
UCHL1 |
PPC |
|
H |
BMI |
CSPG4 |
ERCC2 |
GAPDHS |
ITGA6 |
MCAM |
OAS1 |
PMEL |
SHC4 |
TM4SF1 |
WNT4 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK012 |
|
产地 |
美国 |
|
缩写 |
GQH-MEL |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
上海中乔新舟生物科技有限公司
