小鼠MMP-2ELISA试剂盒描述:
Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme. ScienCell's mouse MMP-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse MMP-2-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse MMP-2 captured in strips.
产品使用说明:仅供科研研究使用
货号 |
EK0460 |
产地 |
美国 |
缩写 |
mMMP-2-ELISA |
规格 |
96 tests |
用途 |
科研 |
保存 |
频繁使用存储在4°C,很少使用存储在-20°C |
运输 |
胶冰 |
参考文献 |
1. Lee, J. G.; Dahi, S.; Mahimkar, R.;
Tulloch, N. L.; Alfonso-Jaume, M. A.; Lovett, D. H.; Sarkar, R. Intronic
regulation of matrix metalloproteinase-2 revealed by in vivo transcriptional
analysis in ischemia. Proc. Nat. Acad. Sci. 102: 16345-16350, 2005. |
2. Morgunova, E.; Tuuttila, A.; Bergmann, U.; Isupov, M.; Lindqvist, Y.; Schneider, G.; Tryggvason, K. Structure of human pro-matrix metalloproteinase-2: activation mechanism revealed. Science 284: 1667-1670, 1999.
3. Huhtala, P.; Eddy, R. L.; Fan, Y. S.; Byers, M. G.; Shows, T. B.; Tryggvason, K. Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16. Genomics 6: 554-559, 1990.
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