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人肝细胞
英文名:Human Hepatocytes
货号:5200
价格:¥14382.00
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  • 参考文献
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人肝细胞说明:


       哺乳动物的肝脏完成许多重要生理过程。不管在生理还是病理条件下,它都是能量代谢、外源性化学物质生物转化和血浆蛋白合成最重要的器官。原代培养人肝细胞是很好的实验模型,在过去和现在都是研究肝脏特定功能的重要工具。用繁殖的人肝细胞进行细胞移植、基因治疗和用于生物人工肝支持系统的肝细胞培养都在研究中。在合适的培养条件下,培养的人类肝细胞增殖并保持已分化的肝细胞功能,比如血清蛋白的合成。    

        人肝细胞(HH)提取于人肝脏组织,于原代末期冻存。每管含有细胞数>1×106cells/ml,此细胞通过albumin, Cytokeratin-18 and Vimentin免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。

    

配套培养基: 肝细胞培养基(HM, Cat. No. 5201)


产品使用说明:仅供科研研究使用


货号 5200
产地 美国
缩写 HH
规格 1 x 10^6 cells/vial, 2 x 10^6 cells/vial
用途 科研
运输 干冰
保存 液氮

1.) Soronen, J., Yki?J�rvinen, H., Zhou, Y., S�devirta, S., Sarin, A., Leivonen, M., Sevastianova, K., Perttil�, J., Laurila, P., Sigruener, A., Schmitz, G. & Olkkonen, V.M. (2016) Novel hepatic microRNAs upregulated in human nonalcoholic fatty liver disease Physiol Rep. 4


2.) Yuan, J., Li, W., Huang, J., Guo, X., Li, X., Lu, X., Huang, X. & Zhang, H. (2015) Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane Stem Cell Res Ther. 6

3.) Xiao, H., Bid, H.K., Jou, D., Wu, X., Yu, W., Li, C., Houghton, P.J. & Lin, J. (2015) A Novel Small Molecular STAT3 Inhibitor, LY5, Inhibits Cell Viability, Cell Migration, and Angiogenesis in Medulloblastoma Cells* J Biol Chem. 290

4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19

5.) Kong, L., Li, S., Huang, M., Xiong, Y., Zhang, Q., Ye, L., Liu, J., Zhu, X., Sun, R. & Guo, Y. (2015) The Roles of Endoplasmic Reticulum Overload Response Induced by HCV and NS4B Protein in Human Hepatocyte Viability and Virus Replication PLoS One. 10

6.) Feng, Y.M., Feng, C.W., Chen, S.Y., Hsieh, H.Y., Chen, Y.H. & Hsu, C.D. (2015) Cyproheptadine, an antihistaminic drug, inhibits proliferation of hepatocellular carcinoma cells by blocking cell cycle progression through the activation of P38 MAP kinase BMC Cancer. 15

7.) Chiang TS, Yang KC, Chiou LL, Huang GT, Lee HS.(2014) "Enhancement of CYP3A4 Activity in Hep G2 Cells by Lentiviral Transfection of Hepatocyte Nuclear Factor-1 Alpha." PloS one. 9: e94885.

8.) Liu Y, Liu A, Xu Z, Yu W, Wang H, Li C, Lin J. (2011) "XZH-5 inhibits STAT3 phosphorylation and causes apoptosis in human hepatocellular carcinoma cells." Apoptosis. 16: 502-10.

9.) Braconi C, Valeri N, Gasparini P, Huang N, Taccioli C, Nuovo G, Suzuki T, Croce CM, Patel T. (2010) "Hepatitis C virus proteins modulate microRNA expression and chemosensitivity in malignant hepatocytes." Clin Cancer Res. 16: 957-66. 

10.) Hikita H, Takehara T, Shimizu S, Kodama T, Shigekawa M, Iwase K, Hosui A, Miyagi T, Tatsumi T, Ishida H, Li W, Kanto T, Hiramatsu N, Hayashi N. (2010) "The Bcl-xL inhibitor, ABT-737, efficiently induces apoptosis and suppresses growth of hepatoma cells in combination with sorafenib." Hepatology. 52: 1310-21.

11.) Liu Y, Li PK, Li C, Lin J. (2010) "Inhibition of STAT3 signaling blocks the anti-apoptotic activity of IL-6 in human liver cancer cells." J Biol Chem. 285: 27429-39.

12.) Shen Q, Cicinnati VR, Zhang X, Iacob S, Weber F, Sotiropoulos GC, Radtke A, Lu M, Paul A, Gerken G, Beckebaum S. (2010) "Role of microRNA-199a-5p and discoidin domain receptor 1 in human hepatocellular carcinoma invasion." Mol Cancer. 9: 227.

13.) Shimizu S, Takehara T, Hikita H, Kodama T, Miyagi T, Hosui A, Tatsumi T, Ishida H, Noda T, Nagano H, Doki Y, Mori M, Hayashi N. (2010) "The let-7 family of microRNAs inhibits Bcl-xL expression and potentiates sorafenib-induced apoptosis in human hepatocellular carcinoma." J Hepatol. 52: 698-704. 

14.) Yeon JH, Na D, Park JK. (2010) "Hepatotoxicity assay using human hepatocytes trapped in microholes of a microfluidic device." Electrophoresis. 31: 3167-74.

15.) Braconi C, Meng F, Swenson E, Khrapenko L, Huang N, Patel T. (2009) "Candidate therapeutic agents for hepatocellular cancer can be identified from phenotype-associated gene expression signatures." Cancer. 115: 3738-48. 

16.) Huang Y, Song N, Ding Y, Yuan S, Li X, Cai H, Shi H, Luo Y. (2009) "Pulmonary vascular destabilization in the premetastatic phase facilitates lung metastasis." Cancer Res. 69: 7529-37. 

17.) Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST, Patel T. (2007) "MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer." Gastroenterology. 133: 647-58.

18.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.

Question:1
Dear Customer Service, I am Zhonghui Zhang who is working in Dr. Wen-Shu Wu’s lab at the University of Illinois at Chicago (UIC). We previously ordered your HH cells and HH medium 09/29/2008 in Maine Medical Center Research Institute (Our lab moved from MMCRI to UIC in 2013). We would like to order a new HH cells from your company, but we don’t want to order this cell from the same donor. Would you please tell me whether the cells were from different donors? By the way, would you please give us some information about previous HH cells (such as age, gender, race and etc.)? Thank you so much! Zhonghui Zhang, Ph.D Section of Hematology/Oncology, Department of Medicine University of Illinois at Chicago 909 S Wolcott Ave, Room 5140 Chicago, IL 60612 Phone: 312-996-2324 (office) or 207-420-5603 (cell)


Answers:

Posted by Manoj Sharma on Thursday, January 7, 2016
hello,
We need lot number to check donor information. And yes we have other lots from different donors. thanks for your interest
SCIENCELL TEAM

Question:2
Can you provide more detail on the source of these cells and general characteristics other than "HH from ScienCell Research Laboratories are isolated from human liver"? Derived from healthy liver tissue, any particular liver site for selection of cellular material, how derived, normal karyotype?


Answers:

Posted by Karishma Shah on Thursday, August 27, 2015
Hi Jeff,
Thanks for asking! Human Hepatocytes are isolated from healthy liver tissue. We have donor information for each lot, if you would like specific information please email sales@sciencellonline.com or call us at 760-602-8549. Due to tissue limitation, there is no particular site in the liver we get cells from. We use the entire liver tissues we obtain. Please let us know if you have any more questions. Thank you!


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