人主动脉平滑肌细胞说明：
平滑肌细胞（SMC）是导致动脉疾病发展的主要因素。血管SMC向增殖表型转化的能力是血管疾病发展和进展的主要因素之一。最近的研究表明，SMC表达钙通道、ICAM-1和VCAM-1。细胞间黏附分子-1和VCAM-1在SMC上的表达可能参与血管壁的炎症反应，并可能积极参与血管疾病的进展。培养的血管SMC在血管疾病的研究中具有重要作用，可用于确定治疗动脉疾病的新靶点。
人主动脉平滑肌细胞(HASMC)提取于人大动脉组织，原代冻存。每管含有细胞数>5×105cells/ml，HASMC具有特异性α-平滑肌肌动蛋白和结蛋白抗体的免疫荧光特征。HASMC对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。在科学研究实验室规定的条件下，细胞可以达到15倍增。
推荐培养基: 平滑肌细胞培养基(SMCM, Cat. No. 1101)
1.) Zheng, C.Y., Song, L.L., Wen, J.K., Li, L.M., Guo, Z.W., Zhou, P.P., Wang, C., Li, Y.H., Ma, D. & Zheng, B. (2015) Tongxinluo (TXL), a Traditional Chinese Medicinal Compound, Improves Endothelial Function After Chronic Hypoxia Both In Vivo and In Vitro J Cardiovasc Pharmacol. 65
2.) Park, S.H., Sung, Y.Y., Nho, K.J. & Kim, H.K. (2015) Protective activity ethanol extract of the fruits of Illicium verum against atherogenesis in apolipoprotein E knockout miceBMC Complement Altern Med. 15
3.) Pahl, M.C., Erdman, R., Kuivaniemi, H., Lillvis, J.H., Elmore, J.R. & Tromp, G. (2015) Transcriptional (ChIP-Chip) Analysis of ELF1, ETS2, RUNX1 and STAT5 in Human Abdominal Aortic Aneurysm Int J Mol Sci. 16
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Koo, H.J., Sohn, E.H., Pyo, S., Woo, H.G., Park, D.W., Ham, Y.M., Jang, S.A., Park, S.Y. & Kang, S.C. (2015) An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-�-stimulated human aortic smooth muscle cells through the NF-?B pathway Int J Mol Med. 35
6.) Guo T, Fang M, Zhang D, Li X. (2013) "Combination treatment with asiaticoside and rapamycin: A new hope for in?stent restenosis." ExpTher Med.6: 557
7.) Liu F, Zhong H, Liang JY, Fu P, Luo ZJ, Zhou L, Gou R, Huang J. (2010) "Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbf?-1 pathway." J Zhejiang Univ Sci B. 11: 905-11.
8.) Xue JH, Yuan Z, Wu Y, Liu Y, Zhao Y, Zhang WP, Tian YL, Liu WM, Liu Y, Kishimoto C. (2010) "High glucose promotes intracellular lipid accumulation in vascular smooth muscle cells by impairing cholesterol influx and efflux balance." Cardiovasc Res. 86: 141-50.
9.) Geng J, Zhao Z, Kang W, Wang W, Liu G, Sun Y, Zhang Y, Ge Z. (2009) "Hypertrophic response to angiotensin II is mediated by protein kinase D-extracellular signal-regulated kinase 5 pathway in human aortic smooth muscle cells." Biochem Biophys Res Commun. 388: 517-22.
10.) Shin IS, Kim JM, Kim KL, Jang SY, Jeon ES, Choi SH, Kim DK, Suh W, Kim YW. (2009) "Early growth response factor-1 is associated with intraluminal thrombus formation in human abdominal aortic aneurysm." J Am Coll Cardiol. 53: 792-9.
11.) Heo SK, Yun HJ, Noh EK, Park WH, Park SD. (2008) "LPS induces inflammatory responses in human aortic vascular smooth muscle cells via Toll-like receptor 4 expression and nitric oxide production." Immunol Lett. 120: 57-64.
12.) Zhang X, Baughman CB, Kaplan DL. (2008) "In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth." Biomaterials. 29: 2217-27.
13.) Song Y, Qi H, Wu C. (2007) "Effect of 1,25-(OH)2D3 (a vitamin D analogue) on passively sensitized human airway smooth muscle cells." Respirology. 12: 486-94.
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