人纤维环细胞cDNA描述：

       Human Annulus Fibrosus Cell PCR-ready first strand cDNA (HAFC cDNA) is prepared from RNA extracted from Human Annulus Fibrosus Cells by a Qiagen RNeasy kit. 2 ug total RNA is then reverse-transcribed using an Applied Biosystems’ High-Capacity cDNA Reverse Transcription kit. 1 μl cDNA is sufficient for one PCR reaction. cDNA from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.

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