Lysis buffer: Modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 ug/ml aprotinin, 5 ug/ml leupeptin, 1 ug/ml pepstatin, 1 mM Na3VO4, 5 mM NaF). Quality Control: Lysate quality was assured with a NuPAGE 4-12% Bis-Tris Gel stained with Coomassie brilliant blue.

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