大鼠巨噬细胞
巨噬细胞是从循环的骨髓来源的单核细胞中分化出来的。在骨髓和之后的血液和组织中，巨噬细胞经历了一个成熟级联的过程，最终转变为形态和功能成熟的组织巨噬细胞。巨噬细胞的功能是清楚细胞碎片和入侵的病原体。他们吞噬入侵的微生物，清除尸体、损伤的细胞和细胞碎片。通过流式细胞术或免疫组化染色可鉴定出几种细胞表面蛋白的特殊表达，如CD14、CD11b、F4/80(小鼠)/EMR1（人）、MAC-1/MAC-3和CD68，而这些蛋白的表达可以表征巨噬细胞。
ScienceCell实验室的Rma-bm提取自成熟大鼠的骨髓中，原代冻存，干冰运输。每管细胞数量超过1x10^6/ml。细胞经F4/80免疫荧光鉴定。该细胞经检测不含支原体, 细菌, 酵母菌和真菌。该细胞不具备增殖能力，故不建议对细胞长期培养和扩增。
推荐培养基：(MaM, Cat. #1921)
产品使用说明：仅供科研研究使用
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货号
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R1920
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产地
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美国
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缩写
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RMa-bm
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规格
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1 x 10^6 cells/vial
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用途
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科研
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运输
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干冰
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保存
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液氮
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[1]. Martin Cline and Margaret Sumner (1972) Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series Blood 40(1) 62-69.
[2]. Siamon Gordon and Philip Taylor (2005) Monocyte and macrophage heterogeneity. Nature Reviews Immunology 5(12) 953-964.
Question: 1
What does it mean by expanding and long-term culture? My cells do not look healthy after splitting after the first passage from the original stock tube from ScienCell.
Answers:
Posted by Manoj Sharma on Monday, August 17, 2015
Expanding means the cells will continue to proliferate and can be passaged to a larger vessel. Rat macrophages do not proliferate and should not be passaged. Please plan your experimental conditions careful before reviving these cells.
1 Answer
My macrophages can't seem to proliferate past P1. The media doesn't seem to be contaminated and I want to stay with these macrophages because they are adherent. Any suggestions?
Posted by Jenna Gipple, Monday, August 17, 2015 on product Rat Macrophages
Question:
My macrophages can't seem to proliferate past P1. The media doesn't seem to be contaminated and I want to stay with these macrophages because they are adherent. Any suggestions?
Answers:
Posted by Manoj Sharma on Monday, August 17, 2015
As stated in the product sheet, rat macrophages do not proliferate. Please plan your experiment carefully before reviving these cells.
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