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大鼠肝脏星形胶质细胞
英文名:Rat Hepatic Stellate Cells
货号:R5300
价格:¥8370.00
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大鼠肝脏星形胶质细胞


       肝脏星形细胞是小叶内结缔组织细胞,呈现肌成纤维细胞样或脂肪细胞形态。他们参与肝脏细胞外基质的稳态,修复,再生,纤维化以有控制视黄醇的代谢,存储和释放。肝脏受伤后,肝脏星形细胞转化成肌成纤维细胞样,并且是纤维化的肝脏中 I 型胶原的主要来源。另外已发现在疾病状态和肝内门静脉高压的发病过程中,肝星状细胞通过细胞收缩调节肝脏微循环。该细胞也能掌控电压激活型钙离子流动,表达低亲和力的生长因子受体p75,并可在神经生长因子的刺激下导致凋亡。因此,星形细胞激活的分子调控在将来有助于治疗纤维化肝,并且能减少慢性肝病患者的发病率和死亡率。
    
       Sciencell研究实验室的大鼠肝星形细胞是从新生2天大鼠的肝脏组织中分离得到的,原代冻存,干冰运输。每管细胞数量超过5×10^5/ml。 经desmin和α-actin免疫荧光鉴定。本细胞经检测不含支原体、细菌、酵母菌和真菌。细胞可达5倍增增殖。
     
推荐培养基:(SteCM, Cat. #5301


产品使用说明:仅供科研研究使用

货号 R5300
产地 美国
缩写 RHSteC
规格 5 x 10^5 cells/vial
用途

科研

运输 干冰
保存 液氮
[1] Reynaert H, Thompson MG, Thomas T, Geerts A. (2002) Hepatic stellate cells: role in microcirculation and pathophysiology of portal hypertension. Gut 50:571-581.


[2] Rockey D. C. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver. Semin Liver Dis 21(3):337-49.


[3] Oide H, Tateyama M, Wang XE, Hirose M, Itatsu T, Watanabe S, Ochi R, Sato N. (1999) Activated stellate (Ito) cells possess voltage-activated calcium current. Biochim. Biophys. Acta. 1418:158-164.


[4] Trim N, Morgan S, Evans M, Issa R, Fine D, Afford S, Wilkins B, Iredale J. (2000) Hepatic stellate cells express the low affinity nerve growth factor receptor p75 and undergo apoptosis in response to nerve growth factor stimulation. Am. J. Pathol. 156(4):1235-1243.

Question:


As stellate cells may get activated if we allow them to multiply, would you recommend to expand the cell line if someone wants to work with quiescent stellate cells?
Answers:

Posted by Manoj Sharma on Monday, January 25, 2016
Rat Hepatic Stellate Cell quiescence and activation is a complicated matter. In general, cells expressing high levels of alpha-smooth muscle actin should be activated and cell not expressing alpha smooth muscle actin should be quiescent. Stellate cells are also able to switch from the activated state back to the quiescent state under certain circumstances. The stellate cells are known as reverted stellate cells at this time and this is an intermediate phenotype.  
I would recommend using the cells as quickly as possible for experiments, however the cells will be proliferative. If you need a completely quiescent state I would recommend reading the following paper as it indicates how to revert hepatic stellate cells back to the quiescent state:
El Taghdouini A, Najimi M, Sancho-Bru P, Sokal E, van Grunsven LA. 2015. “In vitro reversion of activated primary human hepatic stellate cells.” Fibrogenesis Tissue Repair 8:14.
Thank you for your interest.
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