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ScienCell 实验室的MBVP分离自成熟小鼠的大脑中，一代冻存，冰冻运输。每管细胞密度超过5×105/ml。该细胞经Neural/glial antigen 2 (NG2) 和α-平滑肌肌动蛋白的特异抗体免疫荧光学鉴定。本细胞经检测不含支原体、细菌、酵母菌和真菌。
推荐使用培养液：周边细胞培养基-小鼠（PM-m，NO.1231）
产品使用说明：仅供科研研究使用
| 货号 |
M1200 |
| 产地 | 美国 |
| 缩写 |
MBVP |
| 规格 |
5 x 10^5 cells/vial |
| 用途 | 科研 |
| 运输 | 干冰 |
| 保存 | 液氮 |
No Publication available at this time.
Dear Sir, I have isolated pericytes from mouse using pdgfreceptor beta and have maintained them in culture using the pericyte mouse medium from sciencell . I would like to validate the cells for pdgf receptor beta antibody using immunostaining. however the antibody or immunostaining for cells does not seem to work . I saw the pdgf receptor beta antibody immunostaining for mouse brain pericytes done in your cite. I would like to know from you which company (lot no.) pdgf receptor beta antibody is used, corresponding dilution and key steps involved in the staining. The antibodies we have does not seem to work at all for cells but works quite good for tissues. Hence I would be really thankful if you can send across the protocol with antibody details. Thanks Malini
Dear Malini,
The antibody for PDGF-beta receptor that we use here is from Millipore, Cat. No. is 04-397. The dilution we did is 1:200. But if this is a new antibody for your lab, you may want to try different dilutions for the antibody to find the best concentration for your cells. And we use methanol to fix the cells (fix at room temperature for less than 2 min).
SCIENCELL TEAM
上海中乔新舟生物科技有限公司
