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名称 |
BS-C-1非洲绿猴肾细胞系（种属鉴定） |
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货号 |
ZQ0504 |
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产品介绍 |
BSC-1细胞建系于1961年（Hopps，HE，etal）。它对1型脊髓灰质炎病毒（Poliovirustype1），SV40病毒（SV40virus），水泡性口炎病毒（Vesicularstomatitisvirus）敏感，是病毒诊断研究的有用材料。另外，BSC-1细胞还是病毒转染，尤其是SV40病毒载体转染的理想宿主。 |
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种属 |
非洲绿猴 |
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组织来源 |
原位;肾 |
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形态学 |
上皮样 |
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细胞类型 |
自发永生化细胞系 |
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倍增时间 |
72 hours (Note=At 68th passage) (PubMed=14071033) |
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生长方式 |
贴壁 |
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培养基和添加剂 |
MEM（含NEAA）（品牌：中乔新舟 货号：ZQ-300）+10%FBS（品牌：中乔新舟 货号：ZQ500-A）+1%P/S（中乔新舟 货号：CSP006） |
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推荐完全培养基货号 |
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生物安全等级 |
BSL-1 |
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培养条件 |
95%空气，5%二氧化碳；37℃ |
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保藏机构 |
ATCC; CCL-26 BCRC; 60078 CCTCC; GDC0033 CLS; 305009 ECACC; 85011422 IZSLER; BS CL 9 JCRB; IFO50413 JCRB; JCRB9126 KCLB; 10026 NCBI_Iran; C484 |
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供应限制 |
仅供科研使用 |
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货号 |
ZQ0504 |
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发货规格 |
活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一 |
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发货形式 |
活细胞：常温运输；冻存管：干冰运输 |
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储存温度 |
活细胞：培养箱；冻存管：液氮罐 |
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产地 |
中国 |
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供应限制 |
仅供科研使用 |
PubMed=14473108; DOI=10.4049/jimmunol.88.6.796
Meyer H.M. Jr., Hopps H.E., Rogers N.G., Brooks B.E., Bernheim B.C., Jones W.P., Nisalak A., Douglas R.D.
Studies on simian virus 40.
J. Immunol. 88:796-806(1962)
PubMed=14071033; DOI=10.4049/jimmunol.91.3.416
Hopps H.E., Bernheim B.C., Nisalak A., Tjio J.H., Smadel J.E.
Biologic characteristics of a continuous kidney cell line derived from the African green monkey.
J. Immunol. 91:416-424(1963)
PubMed=14215895
Schmidt N.J., Lennette E.H., Shon C.W., Dennis J.
The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses.
Am. J. Public Health Nations Health 54:1522-1530(1964)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=4203458
Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)
PubMed=303774; DOI=10.1073/pnas.74.11.5046
Holley R.W., Armour R., Baldwin J.H., Brown K.D., Yeh Y.-C.
Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by serum factors.
Proc. Natl. Acad. Sci. U.S.A. 74:5046-5050(1977)
PubMed=272650; DOI=10.1073/pnas.75.1.339
Holley R.W., Armour R., Baldwin J.H.
Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by low molecular weight nutrients.
Proc. Natl. Acad. Sci. U.S.A. 75:339-341(1978)
PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)
PubMed=28046048; DOI=10.1371/journal.pone.0169391
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)
PubMed=33458215; DOI=10.1080/23802359.2020.1827067
Kozhukhar N., Alexeyev M.F.
The mitochondrial genome sequence of the BS-C-1 cell line is at odds with the reported derivation from Cercopithecus aethiops.
Mitochondrial DNA B. Resour. 5:3492-3494(2020)
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