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名称 |
B16-F1小鼠黑色素瘤细胞系 |
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货号 |
ZQ0507 |
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产品介绍 |
B16-F1细胞是B16-F0细胞的亚系，源自黑色素瘤C57BL/6J荷瘤小鼠的皮肤；B16-F1细胞可产生黑色素。 |
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种属 |
小鼠 |
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组织来源 |
皮肤组织 |
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形态学 |
上皮细胞样 |
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细胞类型 |
癌细胞系 |
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倍增时间 |
22 hours (PubMed=1109790) |
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生长方式 |
贴壁 |
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培养基和添加剂 |
MEM（含NEAA）培养基(中乔新舟 货号:ZQ-300)+10%FBS（品牌：中乔新舟 货号：ZQ500-A）+1%P/S（中乔新舟 货号：CSP006） |
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推荐完全培养基货号 |
ZM0507 |
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生物安全等级 |
BSL-1 |
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培养条件 |
95%空气，5%二氧化碳；37℃ |
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保藏机构 |
ATCC；CRL-6323 BCRC；60030 BCRJ；0045 CCTCC；GDC0039 CLS；400122 ECACC；92101203 KCLB；80007 NCI-DTP；B16F1 RCB；RCB2649 TKG；TKG 0347 |
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供应限制 |
仅供科研使用 |
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货号 |
ZQ0507 |
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发货规格 |
活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一 |
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发货形式 |
活细胞：常温运输；冻存管：干冰运输 |
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储存温度 |
活细胞：培养箱；冻存管：液氮罐 |
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产地 |
中国 |
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供应限制 |
仅供科研使用 |
1.PubMed=1109790
Fidler I.J.
Biological behavior of malignant melanoma cells correlated to their survival in vivo.
Cancer Res. 35:218-224(1975)
2.PubMed=210294; DOI=10.1093/jnci/61.2.523
Niles R.M., Makarski J.S.
Control of melanogenesis in mouse melanoma cells of varying metastatic potential.
J. Natl. Cancer Inst. 61:523-526(1978)
3.PubMed=6498800; DOI=10.1016/0304-3835(84)90080-6
Wosko T.J., Ferrara D.T., Sartori L.S.
Histological comparison of the B16 melanoma and its F1 variant.
Cancer Lett. 24:57-63(1984)
4.PubMed=6539703; DOI=10.1016/0277-5379(84)90015-4
Weinreb A., Travo P.
Discrimination between human melanoma cell lines by fluorescence anisotropy.
Eur. J. Cancer Clin. Oncol. 20:673-677(1984)
5.PubMed=11833741; DOI=10.1016/S0024-3205(01)01454-0
Nakamura K., Yoshikawa N., Yamaguchi Y., Kagota S., Shinozuka K., Kunitomo M.
Characterization of mouse melanoma cell lines by their mortal malignancy using an experimental metastatic model.
Life Sci. 70:791-798(2002)
6.PubMed=22797548; DOI=10.3892/ijo.2012.1548
Liersch R., Shin J.W., Bayer M., Schwoppe C., Schliemann C., Berdel W.E., Mesters R., Detmar M.
Analysis of a novel highly metastatic melanoma cell line identifies osteopontin as a new lymphangiogenic factor.
Int. J. Oncol. 41:1455-1463(2012)
7.PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)
8.PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)
9.PubMed=33596583; DOI=10.1159/000513174
Piaszinski K., Rincic M., Liehr T., Azawi S.
Molecular cytogenetic characterization of the murine melanoma cell lines S91 clone M3 and B16-F1 with variant B16-4A5.
Cytogenet. Genome Res. 161:82-92(2021)
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