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PK-15猪肾细胞系[套餐促销]
英文名:PK-15
货号:ZQ0516
价格:¥1000.00
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推荐组合

PK-15猪肾细胞系[套餐促销]

¥1000.00
+

PK-15猪肾细胞系专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥1350 ¥1480.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

PK-15猪肾细胞系(种属鉴定)

货号

ZQ0516

产品介绍

PK-15细胞建系于1955(Stice,E)。是PK-1a细胞的克隆系。该细胞系可用于多种病毒的增值及特性研究。另外,电镜观察发现,PK-15细胞内有C-型病毒颗粒存在,是研究C-型病毒的材料。

种属

组织来源

形态学

上皮样

细胞类型

自发永生化细胞系

倍增时间

12.1 +- 0.7 hours (PubMed=17889922); ~25 hours (DSMZ=ACC-640)

生长方式

贴壁

培养基和添加剂

MEM(品牌:中乔新舟  货号:ZQ-300+10%FBS(中乔新舟  货号:ZQ500-A+1%双抗(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0516

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37

保藏机构

ATCC; CCL-33

BCRC; 60057

BCRJ; 0355

CCLV; CCLV-RIE 0005

CCTCC; GDC0061

CLS; 607426

DSMZ; ACC-640

IZSLER; BS CL 72

JCRB; JCRB9040

KCB; KCB 2010002YJ

KCLB; 10033

RCB; RCB0534

供应限制

仅供科研使用


货号

ZQ0516

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


PubMed=5962858
Pirtle E.C.
Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.
Am. J. Vet. Res. 27:747-749(1966)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X
Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.
Characterization of a type C virus released from the porcine cell line PK(15).
Virology 58:65-74(1974)

PubMed=4151202
Tischer I., Rasch R., Tochtermann G.
Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.
Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)

PubMed=6160900
Fulton R.W., Pearson N.J.
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)

PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3009166; DOI=10.1507/endocrj1954.32.819
Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A., Fujita T.
Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
Endocrinol. Jpn. 32:819-828(1985)

PubMed=3173126
Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica 11:263-264(1988)

PubMed=2686830
Dulac G.C., Afshar A.
Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
Can. J. Vet. Res. 53:431-433(1989)

PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005
Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.
Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
J. Virol. 79:3846-3850(2005)

PubMed=17889922; DOI=10.1016/j.virol.2007.08.014
Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K., Kwang J.H.-S.
Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.
Virology 369:423-430(2007)

PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x
Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.
Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.
Int. J. Immunogenet. 36:231-234(2009)

PubMed=19937495; DOI=10.1080/10495390903139950
Uebing-Czipura A.U., Dawson H.D., Rutherford M.S., Scherba G.
Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb.
Anim. Biotechnol. 20:186-215(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24398227; DOI=10.1016/j.vetmic.2013.12.001
Lithgow P.E., Takamatsu H., Werling D., Dixon L.K., Chapman D.
Correlation of cell surface marker expression with African swine fever virus infection.
Vet. Microbiol. 168:413-419(2014)

PubMed=26456528; DOI=10.1126/science.aad1191
Yang L.-H., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J., Shrock E., Xu W.-H., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A., Church G.M.
Genome-wide inactivation of porcine endogenous retroviruses (PERVs).
Science 350:1101-1104(2015)

PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682
Li Y.-Y., Wang Y.-J., Zhou X.-H., Zhang X.-K., Zhang X.-Y., Xia X.-L., Sun H.-C.
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
J. Virol. Methods 271:113682.1-113682.6(2019)

PubMed=32258794; DOI=10.2478/jvetres-2020-0020
Yin M., Hu D.-F., Li P., Kong L.-Y., Ning H.-M., Yue F., Jiang J.-Q., Wang X.-N.
Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus.
J. Vet. Res. 64:9-14(2020)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)

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