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KG-1急性骨髓性白血病细胞(STR鉴定)
英文名:KG-1
货号:ZQ0537
价格:¥1350.00
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KG-1急性骨髓性白血病细胞(STR鉴定)

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KG-1急性骨髓性白血病细胞专用培养基

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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

KG-1急性骨髓性白血病细胞

货号

ZQ0537

产品介绍

KG-1是一种急性骨髓性白血病(AML)细胞系,最初由H.P. Koeffler和D.W. Golde从一位59岁白人男性的骨髓抽取物中建立,该男性最初患有红白血病,后来发展为急性粒细胞性白血病。细胞在形态上类似急性髓原白血病,表现为明显的多形性,骨髓母细胞和骨髓细胞占优势。KG-1细胞少量是成熟的粒细胞,也有微量的巨噬细胞和嗜曙红细胞。KG-1 髓母细胞在佛波醇酯存在下可分化为巨噬细胞。它们对集落刺激因子 (CSF) 有良好的反应。该细胞系为 EBNA 阴性 (EBNA-)。
在急性髓性白血病研究中,KG-1细胞系得到以下应用:耐药性研究、细胞分化研究、基因和蛋白质表达研究、细胞信号传导研究、细胞凋亡研究、白血病干细胞研究、药物筛选和毒性测试、基因突变或异常表达研究、信号通路异常研究、免疫细胞异常研究等。

种属

性别/年龄

男/59岁

组织

骨;骨髓

疾病

急性骨髓性白血病(NCIt:C9154)

细胞类型

肿瘤细胞 

形态学

淋巴母细胞样

生长方式

悬浮

倍增时间

大约38~45小时 

培养基和添加剂

IMDM(品牌:中乔新舟  货号:ZQ-900)+20%FBS(品牌:中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0537

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
TH01: 7,8
TPOX: 7,9
vWA: 14,19
D3S1358: 15,16
D21S11: 28,29
D18S51: 10.2,18
Penta_E: 7,13
Penta_D: 8,9
D8S1179: 13,14
FGA: 22
D19S433: 11,16.2
D2S1338: 20,23

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; CCL-246 ATCC; CRL-8031 BCRC; 60158 BCRJ; 0132 DSMZ; ACC-14 ECACC; 86111306

供应限制

仅供科研使用

货号

ZQ0537

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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