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TF-1人血液白血病细胞 (STR鉴定)
英文名:TF-1
货号:ZQ0577
价格:¥1800.00
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推荐组合

TF-1人血液白血病细胞 (STR鉴定)

¥1800.00
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TF-1人血液白血病细胞专用培养基

¥950.00

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¥2750 ¥2280.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

TF-1人血液白血病细胞

货号

ZQ0577

产品介绍

TF-1细胞系由T. Kitamura等人于1987年10月从患有严重全血细胞减少症的35岁日本男性的肝素化骨髓抽吸样品中建立。

注意事项:
(1)细胞除首次传代需离心收集细胞,后续细胞换液或传代时建议使用半换液法,即换液或传代时待细胞自然沉降后,弃去一半旧培养
液,之后可直接向培养瓶中添加等体积的新鲜培养液,然后将细胞吹打均匀或移入两个新的T25 培养瓶中(1:2传代),补充新的完全
培养基至8-10ml/瓶即可。
(2)细胞低密度时细胞有粘附现象,高密度时细胞为全悬浮状态。
(3)细胞对胎牛血清质量比较敏感,建议使用高质量胎牛血清。

种属

性别/年龄

男/35岁

组织

骨;骨髓

疾病

红白血病

细胞类型

成红血球细胞

形态学

成淋巴细胞

生长方式

悬浮

倍增时间

大约22~70小时  

培养基和添加剂

RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%FBS(中乔新舟  货号:ZQ500-A)+1%P/S(中乔新舟  货号:CSP006+2ng/ml rhGM-CSF

推荐完全培养基货号

ZM0577

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y
D5S818:13,13
D13S317: 8,9
D7S820: 12,12
D16S539: 9,12
vWA: 15,17
TH01: 7,9
TPOX: 8,8
CSF1PO: 13,13
D19S433: 14,16
D21S11: 30,30
D18S51: 13,13 

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 

基因表达

 

保藏机构

ATCC; CRL-2003 DSMZ; ACC-334 ECACC; 93022307

供应限制

仅供科研使用

货号

ZQ0577

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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