产品名称
|
ARH-77人外周血淋巴细胞
|
货号
|
ZQ0582
|
产品介绍
|
ARH-77细胞系是来自患有IgG浆细胞白血病的33岁女性患者的外周血的人类细胞系,已被证明是EBV转化的B淋巴母细胞样细胞系。浆细胞白血病是一种影响骨髓中浆细胞的癌症。
ARH-77细胞的特征在于它们的B淋巴母细胞样表型,这使得它们特别适用于研究B细胞成熟和功能以及浆细胞白血病病理学。并经常用于与爱泼斯坦-巴尔病毒(EBV)相关的研究、多发性骨髓瘤和浆细胞白血病的研究,特别是在药物筛选和治疗靶点的探索中。适用于研究免疫学和相关免疫系统疾病的研究。
|
种属
|
人
|
性别/年龄
|
女/33岁
|
组织
|
外周血
|
疾病
|
浆细胞白血病
|
细胞类型
|
肿瘤细胞
|
形态学
|
成淋巴细胞
|
生长方式
|
悬浮;多细胞聚集体
|
倍增时间
|
大约30~110.4小时
|
培养基和添加剂
|
RPMI-1640基础培养基(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006)+1% Sodium Pyruvate 100 mM Solution(中乔新舟 货号:CSP003)
|
推荐完全培养基货号
|
ZM0582
|
生物安全等级
|
BSL-2
|
STR位点信息
|
Amelogenin:X;
CSF1PO: 6,10;
D2S1338:17;
D3S1358:16;
D5S818: 10,13;
D6S1043:11,19;
D7S820: 7,12;
D8S1179:14,15;
D12S391:19,20;
D13S317:11,13;
D16S539:9,13;
D18S51: 14,16;
D19S433:14,15;
D21S11: 29,30;
FGA:20,21;
Penta D: 10;
Penta E: 12;
TH01:8,9.3;
TPOX:8;
vWA:17;
|
培养条件
|
95%空气,5%二氧化碳;37℃
|
抗原表达/受体表达
|
***
|
基因表达
|
***
|
保藏机构
|
ATCC; CRL-1621 BCRC; 60385 DSMZ; ACC-512 ECACC; 88121201
|
供应限制
|
仅供科研使用
|
货号
|
ZQ0582
|
发货规格
|
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一
|
发货形式
|
活细胞:常温运输;冻存管:干冰运输
|
储存温度
|
活细胞:培养箱;冻存管:液氮罐
|
产地
|
中国
|
供应限制
|
仅供科研使用
|
PubMed=566614
Burk K.H., Drewinko B., Trujillo J.M., Ahearn M.J.
Establishment of a human plasma cell line in vitro.
Cancer Res. 38:2508-2513(1978)
PubMed=7140810; DOI=10.1002/eji.1830120804
Edwards P.A.W., Smith C.M., Neville A.M., O'Hare M.J.
A human-hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line.
Eur. J. Immunol. 12:641-648(1982)
PubMed=6605913; DOI=10.1016/0165-2478(83)90049-4
Ralph P., Saiki O., Maurer D.H., Welte K.
IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester.
Immunol. Lett. 7:17-23(1983)
PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)
PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)
PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)
PubMed=4042077; DOI=10.1002/1097-0142(19851115)56:10<2544::AID-CNCR2820561039>3.0.CO;2-g
Ralph P.
The human B-cell lineage cell line ARH-77.
Cancer 56:2544-2545(1985)
Patent=US4730036
Ralph P., Welte K.
Purifications of B-cell inducing factor (BIF) and B-cell growth factor (BGF).
Patent number US4730036, 08-Mar-1988
PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x
Nakano A., Harada T., Morikawa S., Kato Y.
Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.
Acta Pathol. Jpn. 40:107-115(1990)
PubMed=8051085; DOI=10.1016/S0021-9258(17)32120-8
Liebersbach B.F., Sanderson R.D.
Expression of syndecan-1 inhibits cell invasion into type I collagen.
J. Biol. Chem. 269:20013-20019(1994)
PubMed=7579375; DOI=10.1182/blood.V86.10.4001.bloodjournal86104001
Pellat-Deceunynck C., Amiot M., Bataille R., Van Riet I., Van Camp B., Omede P., Boccadoro M.
Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after.
Blood 86:4001-4002(1995)
PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x
Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)
PubMed=10516762; DOI=10.1038/sj.leu.2401510
Drexler H.G., Dirks W.G., MacLeod R.A.F.
False human hematopoietic cell lines: cross-contaminations and misinterpretations.
Leukemia 13:1601-1607(1999)
DOI=10.1007/0-306-46877-8_4
Jernberg-Wiklund H., Nilsson K.
Multiple myeloma cell lines.
(In) Human cell culture. Vol. 3. Cancer cell lines part 3; Masters J.R.W., Palsson B.O. (eds.); pp.81-155; Kluwer Academic Publishers; New York (2000)
PubMed=10936422; DOI=10.1016/S0145-2126(99)00195-2
Drexler H.G., Matsuo Y.
Malignant hematopoietic cell lines: in vitro models for the study of multiple myeloma and plasma cell leukemia.
Leuk. Res. 24:681-703(2000)
PubMed=14555701
Shammas M.A., Shmookler Reis R.J., Akiyama M., Koley H., Chauhan D., Hideshima T., Goyal R.K., Hurley L.H., Anderson K.C., Munshi N.C.
Telomerase inhibition and cell growth arrest by G-quadruplex interactive agent in multiple myeloma.
Mol. Cancer Ther. 2:825-833(2003)
PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)
PubMed=20454443; DOI=10.1155/2010/904767
Uphoff C.C., Denkmann S.A., Steube K.G., Drexler H.G.
Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines.
J. Biomed. Biotechnol. 2010:904767.1-904767.23(2010)
PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)