产品名称 |
RIN-m5f大鼠胰岛细胞 |
货号 |
ZQ0585 |
产品介绍 |
RIN-m5F细胞系是从RIN-m大鼠胰岛细胞系衍生的克隆。细胞产生和分泌胰岛素,并产生左旋多巴脱羧酶(细胞具有“胺前体摄取和脱羧或APUD活性”的标志)。与亲代不同,它们不产生生长抑素。 |
种属 |
大鼠 |
组织来源 |
胰腺 |
形态学 |
上皮样细胞 |
细胞类型 |
胰腺β细胞 |
换液频率 |
2-3次/周 |
生长方式 |
贴壁 |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006 |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-2058 ATCC; CRL-11605 BCRC; 60410 CCTCC; GDC0223 ECACC; 95090402 IBRC; C10101 NCBI_Iran; C526 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0585 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=6295859; DOI=10.2337/diab.31.6.521
Bhathena S.J., Oie H.K., Gazdar A.F., Voyles N.R., Wilkins S.D., Recant L.
Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor.
Diabetes 31:521-531(1982)
PubMed=6129963; DOI=10.1210/endo-112-3-1070
Oie H.K., Gazdar A.F., Minna J.D., Weir G.C., Baylin S.B.
Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor.
Endocrinology 112:1070-1075(1983)
PubMed=6141566; DOI=10.3181/00379727-175-41762
Bhathena S.J., Awoke S., Voyles N.R., Wilkins S.D., Recant L., Oie H.K., Gazdar A.F.
Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones.
Proc. Soc. Exp. Biol. Med. 175:35-38(1984)
PubMed=2550270; DOI=10.1055/s-0029-1210869
Nielsen J.H.
Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells.
Exp. Clin. Endocrinol. 93:277-285(1989)
PubMed=2569024; DOI=10.1677/joe.0.1210479
Lambert D.G., Atkins T.W.
Effects of islet hormones on insulin secretion from cloned B cell lines, HIT-T15 and RINm5F.
J. Endocrinol. 121:479-485(1989)
PubMed=2090331; DOI=10.1016/0922-3371(90)90097-G
Bartholomeusz R.K., Campbell I.L., Harrison L.C.
A2B5-reactive ganglioside expression determines the differentiation stage and capacity of rat insulinoma (RIN) sublines.
Cell Differ. Dev. 32:39-46(1990)
PubMed=2156672; DOI=10.1210/endo-126-4-1927
Bartholomeusz R.K., Campbell I.L., Harrison L.C.
A2B5-reactive ganglioside expression is an index of differentiation in rat insulinoma (RIN) cells.
Endocrinology 126:1927-1933(1990)
PubMed=8697299
Poitout V., Olson L.K., Robertson R.P.
Insulin-secreting cell lines: classification, characteristics and potential applications.
Diabetes Metab. 22:7-14(1996)
PubMed=10417352; DOI=10.1042/bj3410847
Fiskerstrand C.E., Newey P., Ebrahimi B., Gerrard L., Harrison P., McGregor G.P., Quinn J.P.
Novel cell lines for the analysis of preprotachykinin A gene expression identify a repressor domain 3' of the major transcriptional start site.
Biochem. J. 341:847-852(1999)
DOI=10.1016/j.bej.2003.09.013
Ogawa A., Terada S., Saito Y., Kimura T., Yamaguchi A., Miki M.
CrmA and Bcl-2 protect a cell line derived from islet of Langerhans, RIN-5F from hypoxia-induced apoptosis.
Biochem. Eng. J. 20:209-215(2004)
PubMed=22538498; DOI=10.1159/000338793
Grozinsky-Glasberg S., Shimon I., Rubinfeld H.
The role of cell lines in the study of neuroendocrine tumors.
Neuroendocrinology 96:173-187(2012)