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NCI-H209人小细胞肺癌细胞(STR鉴定)
英文名:NCI-H209
货号:ZQ0810
价格:¥1500.00
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推荐组合

NCI-H209人小细胞肺癌细胞(STR鉴定)

¥1500.00
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NCI-H209 [H209]人小细胞肺癌细胞专用培养基

¥650.00

配套完培,组合使用,省时省力!

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细胞套餐惊爆价

¥2150
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

NCI-H209人小细胞肺癌细胞

货号

ZQ0810

产品介绍

该细胞由GazdarAF及其同事于1979年从一名小细胞肺癌患者的骨髓转移灶中分离建立,该骨髓标本的获取先于患者的治疗。该细胞是一种典型的小细胞性肺癌细胞,表达较高水平的4种生化标志:神经特异性烯醇、肌酸激酶脑型同工酶、左旋多巴脱羧酶、铃蟾肽样免疫活性。c-mycDNA序列没有扩增;未发现大的结构DNA的异常;该细胞合成与正常肺相当量的p53mRNA。该细胞以聚集体的形式悬浮生长,只有聚集体中的细胞是有活力的,但是细胞活率无法估计,一般培养基中含有大量的细胞碎片。这些细胞表达一种不磷酸化的异常形式的RB1,显然是由于密码子706的单点突变(Cys-> Phe)造成的。

种属

性别/年龄

男/55岁

组织

骨髓(转移)

疾病

小细胞肺癌

细胞类型

肿瘤细胞 

形态学

上皮的;成团细胞

生长方式

聚集成团悬浮生长(培养液里会有细胞碎片,但不影响细胞生长)

倍增时间

大约50~70小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+20%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0810

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 11

D13S317: 11

D16S539: 9,12

D5S818: 12

D7S820: 9

THO1: 7,9

TPOX: 8

vWA: 18,19

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; HTB-172 BCRC; 60123 

供应限制

仅供科研使用

货号

ZQ0810

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering.
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