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MM.1S人IgA-骨髓瘤细胞 (STR鉴定)
英文名:MM.1S
货号:ZQ0485
价格:¥1800.00
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MM.1S人IgA-骨髓瘤细胞 (STR鉴定)

¥1800.00
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MM.1S人IgA-骨髓瘤细胞专用培养基

¥550.00

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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MM.1S人IgA-骨髓瘤细胞

货号

ZQ0485

产品介绍

MM.1S是一种人IgA-骨髓瘤细胞系,最初是从患有免疫球蛋白Aλ骨髓瘤的42岁黑人女性患者的外周血中分离的B淋巴母细胞建立的,这些细胞具有淋巴母细胞样的形态,并且既可以悬浮生长也可以轻微贴壁。可用于研究多发性骨髓瘤的疾病进展、药物治疗反应以及耐药性机制。对地塞米松敏感。

注意事项:
(1)该细胞同时存在悬浮生长和轻微贴壁状态。
(2)如果培养过程中培养液逐渐变黄,可加入少量新鲜培养液或分瓶。
(3)如果贴壁部分的细胞融合度达到80%以上,需要用细胞刮将贴壁细胞刮下,和悬浮的细胞一并收集离心,1000rpm,5min,去掉上清液后,加入新鲜培养液重悬细胞并分瓶。
(4)贴壁细胞不能过度融合。
(5)该细胞复苏后需培养2周左右时间,才能恢复正常生长状态。

种属

性别/年龄

女/42岁

组织

外周血

疾病

免疫球蛋白Aλ骨髓瘤

细胞类型

B淋巴细胞

形态学

淋巴母细胞

生长方式

半悬浮

倍增时间

大约72~80小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%双抗(中乔新舟  货号:CSP006)+1% Sodium Pyruvate 100 mM Solution(中乔新舟  货号:CSP003) +1%L-alanyl-L-glutamine(中乔新舟   货号:CSP004

推荐完全培养基货号

ZM0485

生物安全等级

BSL-1

STR位点信息

Amelogenin: X
CSF1PO: 9,14
D13S317: 13
D16S539: 12
D5S818: 8,12
D7S820: 10,11
TH01: 7,8
TPOX: 8
vWA: 15,17
D3S1358: 16,17
D21S11: 29,30
D18S51: 11,16
Penta_E: 13,15
Penta_D: 2.2,9
D8S1179: 14
FGA: 20,24
D19S433: 13
D2S1338: 21,22

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

文献报道抗原表达CD25、CD38、CD52和CD59,糖皮质激素受体。 

基因表达

***  

保藏机构

ATCC; CRL-2974

供应限制

仅供科研使用

货号

ZQ0485

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


1、论文标题: Globular C1q Receptor (gC1qR/p32/HABP1) Suppresses the Tumor-Inhibiting Role of C1q and Promotes Tumor Proliferation in 1q21-Amplified Multiple Myeloma
DOI: 10.3389/fimmu.2020.01292
发表时间: 2020-07-14
期刊: Frontiers in Immunology
影响因子: 5.085
货号: ZQ0485
产品名称: MM1S cells

原文链接: https://www.frontiersin.org/articles/10.3389/fimmu.2020.01292/full


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A high-risk signature for patients with multiple myeloma established from the molecular classification of human myeloma cell lines.
Haematologica 96:574-582(2011)


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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)


PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)


PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)


PubMed=25688540; DOI=10.1002/cyto.a.22643
Maiga S., Brosseau C., Descamps G., Dousset C., Gomez-Bougie P., Chiron D., Menoret E., Kervoelen C., Vie H., Cesbron A., Moreau-Aubry A., Amiot M., Pellat-Deceunynck C.
A simple flow cytometry-based barcode for routine authentication of multiple myeloma and mantle cell lymphoma cell lines.
Cytometry A 87:285-288(2015)


PubMed=25877200; DOI=10.1038/nature14397
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A resource for cell line authentication, annotation and quality control.
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PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
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Genome Med. 7:118.1-118.7(2015)


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Cell 166:740-754(2016)


CLPUB00604

Chow S.
Targeted capture and sequencing of immunoglobulin rearrangements in multiple myeloma to enable detection of minimal residual disease.
Thesis MSc (2017); University of Toronto; Toronto; Canada


PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
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PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
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PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
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Leukemia 34:2754-2765(2020)


PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
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