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HCC1395人乳腺癌细胞(STR鉴定)
英文名:HCC1395
货号:ZQ0945
价格:¥1800.00
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HCC1395人乳腺癌细胞(STR鉴定)

¥1800.00
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HCC1395人乳腺癌细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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¥2150 ¥2980.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

HCC1395人乳腺癌细胞

货号

ZQ0945

产品介绍

HCC1395是一种人乳腺癌细胞系,源自一位43岁白人女性的乳腺导管癌组织。这个细胞系由AF Gazdar和AK Virmani于1994年12月14日成功建立,耗时14个月。HCC1395细胞系具备多倍体特征,并展示出BRCA1 5382C突变的纯合状态。同一患者的淋巴母细胞系(HCC1395BL)则为杂合。

HCC1395细胞携带与乳腺癌相关的TP53基因获得性突变和PTEN基因的纯合缺失,显示出与肿瘤发生和进展密切相关的遗传特。此外,该患者有家族性癌症史,患者母亲曾患乳腺癌,进一步表明遗传因素在该病例中的重要性。

HCC1395细胞的遗传和表型特征使其成为研究乳腺癌发病机制、遗传易感性以及潜在治疗靶点的重要模型。这些细胞的Her2-neu表达呈阴性,但p53表达呈阳性。HCC1395上皮细胞特异性标记物上皮糖蛋白2(EGP2)和细胞角蛋白19呈阳性。根据分离者,细胞的雌激素受体(ER)表达呈阴性,孕激素受体(PR)表达呈阴性。

在形态学上,HCC1395细胞系表现为分化不良,并有空泡化现象。它们是贴壁生长的上皮细胞样形态。

种属

性别/年龄

/43岁

组织

乳房;乳腺/导管

疾病

 癌;导管的;TNM第一阶段, 三级

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 11

D13S317: 9

D16S539: 11

D5S818: 11

D7S820: 10,12

TH01: 9.3

TPOX: 11

vWA: 15

细胞类型

上皮淋巴母细胞

形态学

上皮细胞样

生长方式

贴壁

倍增时间

72 hours (PubMed=25984343); 84.07 hours (JWGray panel)

培养基和添加剂

90%RPMI-1640(中乔新舟 货号:ZQ-200+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%双抗(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0945

培养条件

5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC CRL-2324 KCLB 9S1395

供应限制

仅供科研使用

货号

ZQ0945

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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Int. J. Cancer 78:766-774(1998)


PubMed=9865903
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Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
Oncogene 20:1005-1009(2001)


PubMed=12800145; DOI=10.1002/gcc.10218
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Genes Chromosomes Cancer 37:333-345(2003)


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Genome Res. 14:287-295(2004)


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Science 314:268-274(2006)


PubMed=17932254; DOI=10.1126/science.1145720
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The genomic landscapes of human breast and colorectal cancers.
Science 318:1108-1113(2007)


PubMed=19582160; DOI=10.1371/journal.pone.0006146
Kao J., Salari K., Bocanegra M., Choi Y.-L., Girard L., Gandhi J., Kwei K.A., Hernandez-Boussard T., Wang P., Gazdar A.F., Minna J.D., Pollack J.R.
Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery.
PLoS ONE 4:E6146-E6146(2009)


PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)


PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)


PubMed=21778573; DOI=10.3233/BD-2010-0307
Chavez K.J., Garimella S.V., Lipkowitz S.
Triple negative breast cancer cell lines: one tool in the search for better treatment of triple negative breast cancer.
Breast Dis. 32:35-48(2010)


PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)


PubMed=22585861; DOI=10.1158/2159-8290.CD-11-0224
Marcotte R., Brown K.R., Suarez Saiz F.J., Sayad A., Karamboulas K., Krzyzanowski P.M., Sircoulomb F., Medrano M., Fedyshyn Y., Koh J.L.-Y., van Dyk D., Fedyshyn B., Luhova M., Brito G.C., Vizeacoumar F.J., Vizeacoumar F.S., Datti A., Kasimer D., Buzina A., Mero P., Misquitta C., Normand J., Haider M., Ketela T., Wrana J.L., Rottapel R., Neel B.G., Moffat J.
Essential gene profiles in breast, pancreatic, and ovarian cancer cells.
Cancer Discov. 2:172-189(2012)


PubMed=23601657; DOI=10.1186/bcr3415
Riaz M., van Jaarsveld M.T.M., Hollestelle A., Prager-van der Smissen W.J.C., Heine A.A.J., Boersma A.W.M., Liu J.-J., Helmijr J.C.A., Ozturk B., Smid M., Wiemer E.A.C., Foekens J.A., Martens J.W.M.
miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.
Breast Cancer Res. 15:R33.1-R33.17(2013)


PubMed=24162158; DOI=10.1007/s10549-013-2743-3
Prat A., Karginova O., Parker J.S., Fan C., He X.-P., Bixby L.M., Harrell J.C., Roman E., Adamo B., Troester M.A., Perou C.M.
Characterization of cell lines derived from breast cancers and normal mammary tissues for the study of the intrinsic molecular subtypes.
Breast Cancer Res. Treat. 142:237-255(2013)


PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)


PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)


PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)


PubMed=25485619; DOI=10.1038/nbt.3080
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PubMed=25877200; DOI=10.1038/nature14397
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A resource for cell line authentication, annotation and quality control.
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PubMed=26589293; DOI=10.1186/s13073-015-0240-5
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PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
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PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
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PubMed=28287265; DOI=10.1021/acs.jproteome.6b00470
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PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
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PubMed=30971826; DOI=10.1038/s41586-019-1103-9
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PubMed=31068700; DOI=10.1038/s41586-019-1186-3
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PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
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Cell 180:387-402.e16(2020)


PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

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