上皮细胞培养基
上皮细胞培养基是专门为正常人类上皮细胞体外培养设计的最适于其生长的培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类上皮细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态。
上皮细胞培养基包含500 ml基础培养基，10mll胎牛血清（FBS, Cat. No. 0010） 、5ml上皮细胞生长因子（EpiCGS, Cat. No. 4152 ）、5 ml青霉素/链霉素溶液(P/S, Cat. No. 0503) 。
产品使用说明：仅供科研研究使用
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货号
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4101
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产地
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美国
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缩写
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EpiCM
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规格
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500 ml
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用途
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科研
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包装
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瓶装
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保存
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4℃冰箱
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1.) Mandal, A., Shahidullah, M. & Delamere, N.A. (2015) Calcium entry via connexin hemichannels in lens epithelium Exp Eye Res. 132
2.) Chung, B.H., Kim, K.W., Kim, B.M., Doh, K.C., Cho, M.L. & Yang, C.W. (2015) Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction PLoS One. 10
3.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
4.) Cioni M, Mittelholzer C, Wernli M, Hirsch HH. (2013) "Comparing Effects of BK Virus Agnoprotein and Herpes Simplex-1 ICP47 on MHC-I and MHC-II Expression." Clin Dev Immunol. 2013: 626823.
5.) Du T, Zou X, Cheng J, Wu S, Zhong L, Ju G, Zhu J, Liu G, Zhu Y, Xia S. (2013) "Human Wharton
6.) Meeker RB, Bragg DC, Poulton W, Hudson L. (2012) "Transmigration of macrophages across the choroid plexus epithelium in response to the feline immunodeficiency virus." Cell Tissue Res. 347: 443-55.
Question:1
What is the concentration of glucose in this product?
Answers:
Posted by Karishma Shah on Tuesday, January 12, 2016
5.5 mM
Question:2
hi,I want to know whether the culture medium suitable for Rwpe1 and HK2 cells
Answers:
Posted by Manoj Sharma on Thursday, January 7, 2016
Hello,
Our medium is designed to culture primary cells. thanks for your interest
SCIENCELL
Question:3
Would it be possible to have the full media formulation for EpiCM media? I'd like to know the exact components of the base media as certain components may impact my assays. Thanks
Answers:
Posted by Manoj Sharma on Monday, August 17, 2015
We cannot divulge the entire media formulation, as it is proprietary. We can, however, answer questions about specific components.
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