产品名称 |
NCI-H23 人非小细胞肺癌细胞 |
货号 |
ZQ0957 |
产品介绍 |
NCI-H23细胞源于治疗前患者的肺癌。细胞携带K-ras 12突变,p53基因的第246密码子(ATC -%3E ATG,异亮氨酸-%3E蛋氨酸)有突变。有C-myc、L-myc、v-src、v-abl、v-erb B、c-raf 1、Ha-ras、Ki-ras、N-ras RNAs的表达。这些细胞表达PDGF甲乙链、转化生长因子α和β以及表皮生长因子受体(EGFR)的异质性基因表达。NCI-H23表现出高度的c-myc DNA扩增(20倍),但没有检测到c-myc RNA的扩增。细胞角蛋白5+8和18以及波形蛋白染色阳性,但神经丝染色阴性。NCI-H23细胞左旋多巴脱羧酶阴性。 据报道,它们在软琼脂糖中的集落形成效率为9.7%。 |
种属 |
人 |
性别/年龄 |
男/51岁 |
组织 |
肺 |
疾病 |
腺癌;非小细胞肺癌 |
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 TH01: 6 TPOX: 8,9 vWA: 16,17 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮细胞样 |
生长方式 |
贴壁 |
倍增时间 |
38 hours (Note=In RPMI 1640 + 10% FBS), 71 hours (Note=In ACL-3), 57 hours (Note=In ACL-3 + BSA) (PubMed=3940644); 25 hours (PubMed=7718330); 35 hours (PubMed=25984343); 39.1 hours (PubMed=29681454); 38 hours (ATCC=CRL-5800); 33.4 hours (NCI-DTP=NCI-H23) |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%PS(货号:CSP006) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
细胞来源 |
ATCC |
供应限制 |
仅供科研使用 |
货号 |
ZQ0957 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题: NEDD4L-induced ubiquitination mediating UBE2T degradation inhibits progression of lung adenocarcinoma via PI3K-AKT signaling
DOI: 10.1186/s12935-021-02341-9
发表时间: 2021-11-27
期刊: Cancer Cell International
影响因子: 5.722
货号: ZQ0957
产品名称: H23 cells
原文链接: https://cancerci.biomedcentral.com/articles/10.1186/s12935-021-02341-9
论文标题: High expression of 14‑3‑3ơ indicates poor prognosis and progression of lung adenocarcinoma
DOI: 10.3892/ol.2022.13323
发表时间: 2022-05-12
期刊: Oncology Letters
影响因子: 2.967
货号: ZQ0957
产品名称: NCI-H23 cells
原文链接: https://www.spandidos-publications.com/10.3892/ol.2022.13323
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