产品名称 |
NCI-H727人支气管癌细胞 |
货号 |
ZQ0975 |
产品介绍 |
NCI-H727细胞系是一种人类肺癌细胞系,具有上皮形态,源自一位65岁白人女性的肺类癌(carcinoid tumor)组织。这个细胞系由A.F. Gazdar、H.K. Oie、J.D. Minna及其同事在治疗前从组织中分离得到。NCI-H727细胞系是可用的支气管类癌细胞系中分化最好的一种,它们表达易于检测的p53 mRNA水平,与正常肺组织中发现的水平相比。这些细胞能够合成肽类物质neuromedin B(NMB)(0.1 pmol/mg蛋白),但不能合成胃泌素释放肽(GRP)。NCI-H727细胞系分泌的甲状旁腺激素样蛋白是通过蛋白激酶C途径钙刺激的。表皮生长因子(EGF)受体单克隆抗体可以抑制NCI-H727细胞的生长。。
此外,NCI-H727细胞系表达了synaptophysin、CD56、chromograninA和NSE等神经内分泌标记物,通过免疫细胞化学染色显示。同时,这些细胞通过RT-PCR表达了sstr1、2和5的mRNA,并在免疫细胞化学中显示了sstr1、2A和5的蛋白表达。 |
种属 |
人 |
性别/年龄 |
女/65岁 |
组织 |
肺,支气管 |
疾病 |
肺类癌瘤 |
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X
CSF1PO: 11,12
|
细胞类型 |
肿瘤细胞 |
形态学 |
上皮 |
生长方式 |
贴壁生长 |
倍增时间 |
大约41小时 |
培养基和添加剂 |
RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(品牌:中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-5815 ECACC; 94060303 ICLC; HTL97001 KCLB; 90727 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0975 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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