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NCI-H69人小细胞肺癌细胞
英文名:NCI-H69
货号:ZQ0981
价格:¥1500.00
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NCI-H69人小细胞肺癌细胞

¥1500.00
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NCI-H69人小细胞肺癌细胞专用培养基

¥350.00 ¥450.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥1850 ¥2250.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

NCI-H69人小细胞肺癌细胞

货号

ZQ0981

产品介绍

NCI-H69是一种人小细胞肺癌细胞系,源自一位55岁患有小细胞肺癌的白人男性的胸腔积液转移病灶。该细胞系是非整倍体,将在软琼脂中形成集落,并保留小细胞癌形态和超微结构以及 APUD 细胞特征。
细胞聚集生长,因此细胞计数不准确。细胞的细胞角蛋白染色呈阳性。该生产线可适合在摇瓶或旋转瓶系统中生长。N-myc基因被扩增,并有mRNA和蛋白质的表达。C-myc mRNA(而非蛋白质)低水平表达。存在c-myb、v-fes、v-fms、c-raf 1、Ha-ras、Ki-ras和N-ras mRNA的表达。

种属

性别/年龄

男性/55岁

组织

疾病

小细胞肺癌

生物安全等级

BSL-1

STR位点信息

CSF1PO: 10, 12

D13S317: 12

D16S539: 11

D5S818: 11, 13

D7S820: 9

TH01: 8, 9

TPOX: 10

vWA: 16, 17

Amelogenin: XY

细胞类型

 肿瘤细胞

形态学

 聚团悬浮

生长方式

 悬浮

倍增时间

 56.0 hours (PubMed=1662924)

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200H+10%FBS(品牌:中乔新舟 货号:ZQ0500+1%PS(中乔新舟  货号:CSP006)

推荐完全培养基货号

ZM0981

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

c-myb,v-fes,v-fms,c-raf1,Ha-ras,Ki-rasandN-rasmRNA。 

保藏机构

ATCC; HTB-119 ECACC; 91091802 KCLB; 30119 

供应限制

仅供科研使用


货号

ZQ0981

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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Proc. Natl. Acad. Sci. U.S.A. 78:4591-4595(1981)


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Moody T.W., Pert C.B., Gazdar A.F., Carney D.N., Minna J.D.
High levels of intracellular bombesin characterize human small-cell lung carcinoma.
Science 214:1246-1248(1981)


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Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.
J. Natl. Cancer Inst. 73:801-807(1984)


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Rosen S.T., Mulshine J.L., Cuttitta F., Fedorko J., Carney D.N., Gazdar A.F., Minna J.D.
Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.
Cancer Res. 44:2052-2061(1984)


PubMed=2985257
Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)


PubMed=2985258
Gazdar A.F., Carney D.N., Nau M.M., Minna J.D.
Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.
Cancer Res. 45:2924-2930(1985)


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Whang-Peng J., Lee E.C.
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Oncogene amplification and chromosomal abnormalities in small cell lung cancer.
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Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines.
J. Cell Sci. 91:91-108(1988)


PubMed=3335022
Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)


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In vitro evaluation of the potential of aclarubicin in the treatment of small cell carcinoma of the lung (SCCL).
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Oncogenic point mutations in the human retinoblastoma gene: their application to genetic counseling.
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Metabolic activation of 4-ipomeanol in human lung, primary pulmonary carcinomas, and established human pulmonary carcinoma cell lines.
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PubMed=1662924
Khan M.Z., Freshney R.I., Murray A.M.B., Merry S., Plumb J.A., McNicol A.M.
Identification and characterisation in vitro of cells with a non-SCLC cell-like phenotype derived from a continuous SCLC cell line.
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PubMed=1845952; DOI=10.1002/1097-0142(19910201)67:3<619::AID-CNCR2820670317>3.0.CO;2-Y
Broers J.L.V., Mijnheere E.P., Rot M.K., Schaart G., Sijlmans A., Boerman O.C., Ramaekers F.C.S.
Novel antigens characteristic of neuroendocrine malignancies.
Cancer 67:619-633(1991)


PubMed=1847845; DOI=10.1007/BF00685110
Sasaki Y., Shinkai T., Eguchi K., Tamura T., Ohe Y., Ohmori T., Saijo N.
Prediction of the antitumor activity of new platinum analogs based on their ex vivo pharmacodynamics as determined by bioassay.
Cancer Chemother. Pharmacol. 27:263-270(1991)


PubMed=7903701; DOI=10.1093/jnci/86.2.110
Barrand M.A., Heppell-Parton A.C., Wright K.A., Rabbitts P.H., Twentyman P.R.
A 190-kilodalton protein overexpressed in non-P-glycoprotein-containing multidrug-resistant cells and its relationship to the MRP gene.
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PubMed=7736387; DOI=10.1002/1097-0142(19950515)75:10<2442::AID-CNCR2820751009>3.0.CO;2-Q
Campling B.G., Sarda I.R., Baer K.A., Pang S.C., Baker H.M., Lofters W.S., Flynn T.G.
Secretion of atrial natriuretic peptide and vasopressin by small cell lung cancer.
Cancer 75:2442-2451(1995)


PubMed=8626706; DOI=10.1074/jbc.271.19.11477
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Insulin-like growth factor expression in human cancer cell lines.
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PubMed=8712684
Khan M.Z., McNicol A.M., Freshney R.I.
Tumorigenicity, invasion and metastasis of the small cell lung cancer cell line NCI-H69 and two derivative lines MOG-H69V and MOG-H69VZ.
Anticancer Res. 16:1659-1669(1996)


PubMed=8784480; DOI=10.1159/000227600
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Lack of c-mpl proto-oncogene transcripts and growth-stimulatory effects of thrombopoietin on human small cell lung cancer cell lines.
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PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
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PubMed=8806103; DOI=10.1002/jcb.240630516
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MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
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PubMed=9413215
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Small cell lung cancer can express CD34 antigen.
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PubMed=9815546
Campling B.G., Young L.C., Baer K.A., Lam Y.-M., Deeley R.G., Cole S.P.C., Gerlach J.H.
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Clin. Cancer Res. 3:115-122(1997)


PubMed=9559344; DOI=10.1002/(SICI)1098-2264(199804)21:4<326::AID-GCC6>3.0.CO;2-0
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Investigation of the genes for RET and its ligand complex, GDNF/GFR alpha-I, in small cell lung carcinoma.
Genes Chromosomes Cancer 21:326-332(1998)


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PubMed=10037197
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PubMed=10536175; DOI=10.3892/ijo.15.5.927
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