产品名称 |
SU-DHL-4人B细胞淋巴瘤 |
货号 |
ZQ0982 |
产品介绍 |
该细胞系建系于1976年,来自38岁白人男性的腹膜渗出物。这个细胞系代表了弥漫性大b细胞淋巴瘤(DLBCL)的模型,DLBCL是成人中最常见的非霍奇金淋巴瘤之一。该细胞系的建立为DLBCL的生物学,特别是淋巴瘤发生和肿瘤进展的细胞和分子机制提供了有价值的见解。该细胞系有14号、18号染色体易位。在BCL-2基因中有一个主要的重排。该细胞系对念珠菌摄入呈阴性。有资料显示该细胞对爱泼斯坦-巴尔病毒(EBV)呈阴性。每个批次均通过本库支原体检测,结果阴性。
在研究中,SU-DHL-4细胞被广泛用于研究各种化疗药物和靶向治疗药物的疗效和作用机制,反映了其在淋巴瘤治疗研究中的重要性。这些细胞表达一些与b细胞谱系相关的关键免疫表型标记,如CD19和CD20,它们对b淋巴细胞的发育和功能至关重要。这些标记也使SU-DHL-4成为测试b细胞特异性疗法的极好靶点,包括单克隆抗体和小分子抑制剂,它们破坏淋巴瘤细胞存活和增殖的关键信号通路。 注意事项:建议接种密度为10万至20万/ml,建议传代密度为80万 至100万/ml。 |
种属 |
人 |
性别/年龄 |
男性/38岁 |
组织 |
腹腔积液 |
疾病 |
弥漫性大B细胞淋巴瘤 |
生物安全等级 |
BSL-1 |
STR位点信息 |
D5S818: 11, 12 |
细胞类型 |
肿瘤细胞 |
形态学 |
淋巴母细胞样 |
生长方式 |
悬浮 |
倍增时间 |
~40 hours (DSMZ=ACC-495) |
培养基和添加剂 |
RPMI-1640(中乔新舟 货号:ZQ-200)+10%FBS(品牌:中乔新舟 货号:ZQ0500)+1%PS(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2957 DSMZ; ACC-495 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0982 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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