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LN229人大脑神经母瘤细胞 (STR鉴定)
英文名:LN229
货号:ZQ1001
价格:¥1700.00
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LN229人大脑神经母瘤细胞 (STR鉴定)

¥1700.00
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LN229[LN-229; LNT-229]人大脑神经母瘤细胞专用培养基

¥350.00 ¥400.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥2050 ¥2100.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

LN229人大脑神经母瘤细胞

货号

ZQ1001

产品介绍

LN229细胞是一种人类胶质母细胞瘤(glioblastoma multiforme, GBM)细胞系,最初在1979年从一位60岁白人女性患者的右额顶枕胶质母细胞瘤中建立。胶质母细胞瘤是最具侵袭性和致死性的脑癌之一,LN229细胞被广泛用于研究,以了解该疾病的分子基础并制定潜在的治疗策略。细胞表现出上皮样形态和粘附生长特性,这使它成为体外研究的理想选择。考虑到它的高致瘤性,当注射到裸鼠体内时,很容易形成肿瘤,使其成为癌症研究的强大模型。

LN229细胞的关键特征之一是存在突变的p53基因(TP53),在密码子98处具有特异性的CCT (Pro)到CTT (Leu)突变。这种突变对细胞系的攻击行为和对凋亡的抵抗起着重要作用。此外,LN229细胞有一个野生型PTEN基因,但它们在p16和p14ARF肿瘤抑制基因中表现出纯合缺失,而p16和p14ARF是细胞周期和凋亡的重要调节因子。这些遗传改变使LN229成为研究这些突变对肿瘤生物学和治疗耐药性影响的有价值的模型。
LN229细胞在细胞凋亡的研究中尤其有用。它们在Fas配体刺激下发生凋亡,并在16小时内发生细胞死亡。有趣的是,虽然Bcl-2的表达可以保护LN229细胞免受Fas配体诱导的凋亡,但它对嘌呤霉素(一种蛋白质合成抑制剂)诱导的凋亡仅提供有限的保护。这种选择性耐药模式使LN229细胞成为了解胶质母细胞瘤细胞凋亡分子机制和测试潜在细胞凋亡调节疗法的关键模型。

种属

性别/年龄

女性/60岁

组织

/右额叶顶枕皮层

疾病

胶质母细胞瘤

生物安全等级

BSL-1

STR位点信息

Amelogenin:X

CSF1PO:12

D13S317:10,11

D16S539:12

D5S818:11,12

D7S820:8,11

TH01:9.3

TPOX:8

vWA:16,19

细胞类型

胶质母细胞瘤

形态学

上皮样

生长方式

贴壁生长

倍增时间

24 hours (PubMed=9842975); 31 hours (PubMed=25984343)

培养基和添加剂

DMEM(中乔新舟 货号:ZQ-100)+5%FBS(中乔新舟 货号:ZQ500-A)+1%PS(中乔新舟 货号:CSP006

推荐完全培养基货号

ZM1001

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC;CRL-2611

供应限制

仅供科研使用


货号

ZQ1001

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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