配套完培,省时省力,单买细胞无优惠
产品名称 |
LN229人大脑神经母瘤细胞 |
货号 |
ZQ1001 |
产品介绍 |
LN229细胞是一种人类胶质母细胞瘤(glioblastoma multiforme, GBM)细胞系,最初在1979年从一位60岁白人女性患者的右额顶枕胶质母细胞瘤中建立。胶质母细胞瘤是最具侵袭性和致死性的脑癌之一,LN229细胞被广泛用于研究,以了解该疾病的分子基础并制定潜在的治疗策略。细胞表现出上皮样形态和粘附生长特性,这使它成为体外研究的理想选择。考虑到它的高致瘤性,当注射到裸鼠体内时,很容易形成肿瘤,使其成为癌症研究的强大模型。
LN229细胞的关键特征之一是存在突变的p53基因(TP53),在密码子98处具有特异性的CCT (Pro)到CTT (Leu)突变。这种突变对细胞系的攻击行为和对凋亡的抵抗起着重要作用。此外,LN229细胞有一个野生型PTEN基因,但它们在p16和p14ARF肿瘤抑制基因中表现出纯合缺失,而p16和p14ARF是细胞周期和凋亡的重要调节因子。这些遗传改变使LN229成为研究这些突变对肿瘤生物学和治疗耐药性影响的有价值的模型。 |
种属 |
人 |
性别/年龄 |
女性/60岁 |
组织 |
脑/右额叶顶枕皮层 |
疾病 |
胶质母细胞瘤 |
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin:X CSF1PO:12 D13S317:10,11 D16S539:12 D5S818:11,12 D7S820:8,11 TH01:9.3 TPOX:8 vWA:16,19 |
细胞类型 |
胶质母细胞瘤 |
形态学 |
上皮样 |
生长方式 |
贴壁生长 |
倍增时间 |
|
培养基和添加剂 |
DMEM(中乔新舟 货号:ZQ-100)+5%FBS(中乔新舟 货号:ZQ500-A)+1%PS(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC;CRL-2611 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1001 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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